HRP anti-Neurofilament H & M (NF-H/NF-M), Phosphorylated Antibody

Pricing & Availability
Clone
SMI 310 (See other available formats)
Other Names
Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Isotype
Mouse IgG1, κ
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Product Citations
publications
SMI-310_HRP_Neurofilament_Phosphorylated_Antibody_IHC_062017
IHC staining of HRP anti-Neurofilament H & M (NF-H/NF-M), Phosphorylated Antibody (Clone SMI 310) on formalin-fixed paraffin-embedded human cerebellum brain tissue. After antigen retrieval using Retrieve-All Antigen Unmasking System 3, the tissue was incubated with the primary antibody at 1 µg/mL for overnight at 4°C. DAB was used for detection followed by hematoxylin and Bluing solution counterstaining.
  • SMI-310_HRP_Neurofilament_Phosphorylated_Antibody_IHC_062017
    IHC staining of HRP anti-Neurofilament H & M (NF-H/NF-M), Phosphorylated Antibody (Clone SMI 310) on formalin-fixed paraffin-embedded human cerebellum brain tissue. After antigen retrieval using Retrieve-All Antigen Unmasking System 3, the tissue was incubated with the primary antibody at 1 µg/mL for overnight at 4°C. DAB was used for detection followed by hematoxylin and Bluing solution counterstaining.
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837707 25 µg $105
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837708 100 µg $265
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Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and regulate axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE: the light or lowest NF (NF-L) runs at 68-70 kD; the medium or middle NF (NF-M) runs at about 145-160 kD; the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from glia (negative for NF).

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated to HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting4, immunocytochemistry2 and immunohistochemical staining of frozen tissue sections1, 3.

Clone SMI 310 reacts with an extensively phosphorylated epitope of neurofilament H and, to a much lesser extent, neurofilament M in most mammalian species. Phosphatase treatment of samples abolishes reaction with SMI 310. A very extensive degree of hyperphosphorylation of neurofilaments seems to be necessary for its reactivity.

Application References

(PubMed link indicates BioLegend citation)
  1. Dubourg O, et al. 2011. Acta Myol. 30(2):103. (IHC-F) PubMed
  2. Mizui T, et al. 2009. J Neurochem. 109(2):611. (ICC)
  3. Denlger-Crish C, et al. 2014. Front Neursci. 8:290. (IHC-F) PubMed
  4. Mulot SF, et al. 1994. FEBS Lett. 349(3):359. (WB)
RRID
AB_2686950 (BioLegend Cat. No. 837707)
AB_2686951 (BioLegend Cat. No. 837708)

Antigen Details

Structure
The medium or middle neurofilament (NF-M) runs at 145-160 kD, and the heavy or highest neurofilament (NF-H) runs at 200-220 kD.
Distribution

Tissue distribution: CNS, peripheral nerves and glandular cells of the prostate.
Cellular distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion.

Function
Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
Interaction
Cell bodies and dendrites are generally unstained while other cells and tissues are unreactive, except for peripheral axons.
Cell Type
Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments, Phospho-Proteins
Antigen References
  1. Siedler D, et al. 2014. Front Cell Neurosci. 8:429.

 

Gene ID
4744 View all products for this Gene ID
UniProt
View information about Neurofilament H & M (NF-H/NF-M), Phospho on UniProt.org

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Go To Top Version: 2    Revision Date: 01/29/2018

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