HRP anti-Myelin CNPase Antibody

Pricing & Availability
Clone
SMI 91 (See other available formats)
Other Names
CNPase, Cnp-1, Cnp1, 2',3'-cyclic-nucleotide 3'-phosphodiesterase
Isotype
Mouse IgG1, κ
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Product Citations
publications
SMI-91_HRP_Myelin_CNPase_Antibody_1_032318
Western blot of HRP anti-Myelin CNPase antibody (clone SMI 91). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2.5 µg/mL of primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • SMI-91_HRP_Myelin_CNPase_Antibody_1_032318
    Western blot of HRP anti-Myelin CNPase antibody (clone SMI 91). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2.5 µg/mL of primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • SMI-91_HRP_Myelin_CNPase_Antibody_2_032318
    IHC staining of HRP anti-Myelin CNPase antibody (Clone SMI 91) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.E.I.R (Cat. No. 928502), the tissue was incubated with 1 µg/mL of the primary antibody for one hour at room temperature. DAB was used for detection followed by hematoxylin counterstaining and bluing solution counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
836405 25 µg $105
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836406 100 µg $265
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Description

High CNPase expression is seen in myelin producing cells, including oligodendrocytes and Schwann cells. CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (CNS). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth. The enzyme isolated from the mammalian brain is primarily a mixed dimer of approximately 94 kD. The dimer consists of a varied proportion of CNP1 (46 kD) and CNP2 (48 kD) subunits in various species. Since the enzyme is a myelin-associated enzyme, it is of considerable interest in the study of diseases and disorders in which myelin is affected, such as multiple sclerosis, subacute sclerosing panencephalitis, acquired immunodeficiency with CNS involvement, and peripheral neuropathies. The combination of clone SMI 91 with clone SMI 94 and/or clone SMI 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.5 - 5.0 µg per ml. For immunohistochemistry of formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The reactivity of Alexa Fluor® 647 anti-Myelin CNPase antibody was validated in FFPE mouse and rat brain tissue, and the HRP anti-Myelin CNPase antibody was validated in FFPE rat brain tissue.

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections1 (IHC-F)

Application References

(PubMed link indicates BioLegend citation)
  1. Samanta J, et al. 2010. Stroke. 2:357-62. (IHC-F) PubMed
  2. Werner HB, et al. 2007. J. Neurosci. 27:7717-7730. (WB, IHC-P) PubMed
  3. McFerran B, et al. 1997. J Cell Sci. 110:2979-2985. (WB)
RRID
AB_2728548 (BioLegend Cat. No. 836405)
AB_2728549 (BioLegend Cat. No. 836406)

Antigen Details

Structure
CNPase is a 421 amino acid protein with a molecular mass of approximately 48 kD.
Distribution

Tissue distribution: Central and peripheral nervous systems.
Cellular distribution: Cytoskeleton, nucleus, mitochondria, cytosol, and plasma membrane.

Function
CNPase detects developing and adult myelin, and developing oligodendrocytes and Schwann cells. CNPase distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Phospho-Proteins, Enzymes and Regulators
Antigen References
  1. Han H, et al. 2013. Biofactors. 39(3):233.
  2. Raasakka A, et al. 2014. Neurosci Bull. 30(6):956.
Gene ID
1267 View all products for this Gene ID
UniProt
View information about Myelin CNPase on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/05/2017

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