HRP anti-mouse/rat β-Amyloid, 1-16 Antibody

Pricing & Availability
Clone
M3.2 (See other available formats)
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M3dot2_HRP_beta-amyloid_Antibody_1_041118
Western blot of HRP anti-mouse/rat β-Amyloid, 1-16 antibody (clone M3.2). Lane 1: Molecular weight marker; Lane 2: 5 µg of human brain lysate; Lane 3: 5 µg of mouse brain lysate; Lane 4: 5 µg of rat brain lysate. The blot was incubated with 0.005 µg/mL of primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • M3dot2_HRP_beta-amyloid_Antibody_1_041118
    Western blot of HRP anti-mouse/rat β-Amyloid, 1-16 antibody (clone M3.2). Lane 1: Molecular weight marker; Lane 2: 5 µg of human brain lysate; Lane 3: 5 µg of mouse brain lysate; Lane 4: 5 µg of rat brain lysate. The blot was incubated with 0.005 µg/mL of primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • M3dot2_HRP_beta-amyloid_Antibody_2_041118
    Western blot of HRP anti-mouse/rat β-Amyloid, 1-16 antibody (clone M3.2). Lane 1: Molecular weight marker; Lane 2: 30 ng of human Aβ 1-40 peptide; Lane 3: 30 ng mouse/rat Aβ 1-40 peptide. The blot was incubated with 0.025 µg/mL of primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • M3dot2_HRP_beta-amyloid_Antibody_3_041118
    Detection ELISA of HRP anti-mouse/rat β-Amyloid, 1-16 antibody (clone M3.2) binding to rodent beta amyloid 1-42 peptide. ELISA was performed by coating wells with 150 ng of purified anti-mouse/rat β-Amyloid antibody, polyclonal (Cat. No. 805801). The wells were then incubated at 37°C for one hour with serially diluted (5 µg/ml to 0.005 µg/mL) rodent Aβ 1-42 peptide, followed by detection with HRP anti-mouse/rat β-Amyloid, 1-16 antibody (clone M3.2). TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system (Cat. No. 421501).
Cat # Size Price Quantity Avail. Save
805705 25 µg $105
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805706 100 µg $260
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Description

Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The epitope has been determined to be within residues 10-15 of murine Aβ.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
ELISA Detection - Validated
ELISA Capture, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.005-0.01 µg per ml. For ELISA Detection applications, a concentration range of 1.0-5.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone M3.2 recognizes a rodent sequence of Aβ, β-CTF, sAPP-α, and full length APP. Clone 3.2 has negligible cross reactivity with human isoforms of APP and beta amyloid.

 

This antibody is effective in immunoblotting (WB), and ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Morales-Corraliza J, et al.  2009. PLoS One 4(9):e7134. (IP, WB, ELISA Detection)
  2. Teich AF, et al. 2013. PLoS One 8(2):e55647, 2013. (ELISA Capture, WB)

 

RRID
AB_2728532 (BioLegend Cat. No. 805705)
AB_2728533 (BioLegend Cat. No. 805706)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue sources: Primarily nervous system, but also adipose tissue, intestine, muscle.

Distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1.
  3. Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
  4. Puig KL, Combs CK. 2013. Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ, Hardy J. 2016. EMBO Mol. Med. 8(6):595.
  6. Walsh DM, Selkoe DJ. 2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid, 1-16 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/12/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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