- Clone
- SMI 52 (See other available formats)
- Other Names
- Microtubule-associated protein 2, MAP-2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IHC staining of HRP anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1x (Cat. No. 928502), the tissue was incubated with 5 µg/mL of the primary antibody for 60 minutes at room temperature. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of HRP anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1x (Cat. No. 928502), the tissue was incubated with 5 µg/mL of the primary antibody for 60 minutes at room temperature. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 801808 | 25 µg | $105 | ||||
| 801809 | 100 µg | $265 | ||||
Microtubules are 25nm diameter protein rods found in most kinds of eukaryote cells. They are polymerized from a dimeric subunit made of one α subunit and one β tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5.
Product DetailsProduct Details
- Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against Microtubule-Associated Protein (MAP2)
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for relevant formats) include: immunocytochemistry5,6, ELISA7, and WB1,5.
SMI 52 reacts with microtubule-associated protein 2 (MAP2). This antibody reacts with most mammalian species and with frog tissue. SMI 52 recognizes neuronal cell bodies and dendrites in tissue sections and cell cultures. In immunoblots of brain cytoskeletal preparations, SMI 52 reacts with MAP2a and b, and also with a doublet at 68 kD that may represent MAP2c. -
Application References
(PubMed link indicates BioLegend citation) -
- Shi Y, et al. 2017. Cell Death Differ. 24(1):167. (WB)
- Shelton MA, et al. 2015. Biol. Psychiatry. 78(6):374. (IHC) PubMed
- Wang X, et al. 2015.PLOS ONE. 10: 0145441. (IHC) PubMed
- Kazim SF, et al. 2014. Neuro Dis. 71:110. (IHC)
- Cardenas-Aguayo MC, et al. 2013. PLOS ONE. (1):e53596. (ICC, WB) PubMed
- Lo Furno D, et al. 2013. J. Cell. Physiol. 228:2109. (ICC)
- Gensel JC, et al. 2009.J. Neursci 29: 3956-3968. (ELISA) PubMed
- RRID
-
AB_2728524 (BioLegend Cat. No. 801808)
AB_2728525 (BioLegend Cat. No. 801809)
Antigen Details
- Structure
- MAP2 is made up of two bands of an apparent molecular weight of approximately 280 kD, referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kD on SDS-PAGE gels. All
- Distribution
-
Cellular distribution: Plasma membrane, cytoskeleton, nucleus, and cytosol
Tissue distribution: Predominantly in the nervous system - Function
- MAP2 encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are involved in microtubule assembly. The products of these genes are neuron-specific cytoskeletal proteins that are enriched in dendrites, which implicate a role in determining and stabilizing dentritic shape during neuron development.
- Interaction
- MAP2 interacts with microtubules. It has also been reported to interact with KNDC1 via KIND2.
- Cell Type
- Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Microtubules
- Antigen References
-
- Zhou Y, et al. 2015. Neuro Oncol. 17(12):1578.
- Loeser H, et al. 2015. Am J Nephron. 41(3):191.
- Gene ID
- 4133 View all products for this Gene ID
- UniProt
- View information about MAP2 on UniProt.org
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