HRP anti-IP3R1 Antibody

Pricing & Availability
Clone
L24/18 (See other available formats)
Other Names
Inositol 1,4,5-trisphosphate receptor type 1, IP-3-R, IP3R 1, IP3 receptor, type 1 InsP3 receptor, inositol 1,4,5-triphosphate receptor 1, inositol 1,4,5-triphosphate receptor, type 1, type 1 inositol 1,4,5-trisphosphate receptor
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
L24-18_HRP_IP3R1_Antibody_1_052918
Western blot of HRP anti-IP3R1 antibody (clone L24/18). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 10 μg of mouse brain lysate; Lane 4: 10 μg of rat brain lysate. The blot was incubated with 1 μg/mL of clone N46/30 overnight at 4°C. HRP anti-β-tubulin antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
  • L24-18_HRP_IP3R1_Antibody_1_052918
    Western blot of HRP anti-IP3R1 antibody (clone L24/18). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 10 μg of mouse brain lysate; Lane 4: 10 μg of rat brain lysate. The blot was incubated with 1 μg/mL of clone N46/30 overnight at 4°C. HRP anti-β-tubulin antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
  • L24-18_HRP_IP3R1_Antibody_2_052918
    IHC staining of HRP anti-IP3R1 antibody (clone L24/18) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 0.2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • L24-18_HRP_IP3R1_Antibody_3_052918
    IHC staining of HRP anti-IP3R1 antibody (clone L24/18) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 0.2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • L24-18_HRP_IP3R1_Antibody_4_052918
    IHC staining of HRP anti-IP3R1 antibody (clone L24/18) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 0.2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
817703 25 µg $100
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817704 100 µg $170
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Description

Inositol 1,4,5-triphosphate (IP3) is a calcium-regulating second messenger that receptor involved in ER stress-induced apoptosis. IP3 receptor, type 1 (IP3R1) is a receptor for IP3 expressed on the ER which opens a calcium channel on the ER after IP3 bidning. IP3R1 is expressed in developing and mature neurons and its dysregulation may be involved in progression of certain neurodegenerative diseases. IP3R1 is also involved in autophagy, cellular energenetics, macrophage inflammasomes, and other cellular processes.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 2680-2749 of rat IP3 receptor type 1.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 0.2 - 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

* Expected MW: 300 kD

This antibody is less reactive in human samples than mouse and rat sample in WB.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhao G, et al. 2010. J. Gen. Physiol. 136(3):283-91 (WB)
RRID
AB_2734570 (BioLegend Cat. No. 817703)
AB_2734571 (BioLegend Cat. No. 817704)

Antigen Details

Structure
IP3R1 is a 2758 amino acid protein with an apparent molecular mass of 314 kD.
Distribution

Cellular distribution: Plasma membrane, endoplasmic reticulum, and nucleus.

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

1. Tang T-S, et al. 2009. J Neurosci. 29(5):1257-66 

Gene ID
25262 View all products for this Gene ID
UniProt
View information about IP3R1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/29/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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