HRP anti-Flotillin-1 Antibody

Pricing & Availability
Clone
W16108A (See other available formats)
Other Names
Flotillin 1, Flot-1, Integral Membrane Component of Caveolae
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16108A_HRP_Flotillin-1_Antibody_022218
Western blot of HRP anti-Flotillin-1 antibody (clone W16108A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysates; Lane 4: 20 µg of rat brain lysates; Lane 5: 20 ng of recombinant MYC/DDK tagged-Flotillin-1; Lane 6: 20 ng of recombinant GST tagged-Flotillin-2. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4µC. Flotillin-1 was visualized using chemiluminescence detection.
  • W16108A_HRP_Flotillin-1_Antibody_022218
    Western blot of HRP anti-Flotillin-1 antibody (clone W16108A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysates; Lane 4: 20 µg of rat brain lysates; Lane 5: 20 ng of recombinant MYC/DDK tagged-Flotillin-1; Lane 6: 20 ng of recombinant GST tagged-Flotillin-2. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4µC. Flotillin-1 was visualized using chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
849803 25 µg $140
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849804 100 µg $305
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Description

The flotillin family is composed of two highly homologous proteins, flotillin-1 and flotillin-2 that share ~50% amino acid sequence similarity. Flotillins are ubiquitously expressed, membrane-associated proteins, and were shown to be associated with lipid microdomains called membrane rafts. Flotillins are present in various cellular compartments, including the plasma membrane and endosomes, and they have been suggested to function in cellular signaling and membrane trafficking processes, including endocytosis and endosomal trafficking.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, and Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Flotillin-1 recombinant protein corresponding to amino acids 1-270 expressed in E. coli.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone specifically recognizes Flotillin-1 but not Flotillin-2, validated using WB.

RRID
AB_2728577 (BioLegend Cat. No. 849803)
AB_2728578 (BioLegend Cat. No. 849804)

Antigen Details

Structure
Flotillin-1 is a 427 amino acid protein with a molecular mass of 47 kD.
Distribution

Tissue distribution: Flotillin-1 is ubiquitously expressed.
Cellular distribution: Flotillin-1 is mainly expressed in the cytosol and endosomes.

Function
Flotillins are thought to interact with the cytoskeleton and function in a number of cellular processes such as endocytosis.
Interaction
Flotillin-1 interacts with cbl-associated protein/ponsin (CAP).
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Antigen References

1. Otto G et al., 2011. Journal of Cell Science. 124: 3933.

Gene ID
10211 View all products for this Gene ID
UniProt
View information about Flotillin-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/23/2018

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