HRP anti-Clusterin Antibody

Pricing & Availability
Clone
A15113A (See other available formats)
Other Names
Apolipoprotein J, Apo-J, Complement cytolysis inhibitor, Aging-associated gene 4 protein, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2 TRPM-2
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A15113A_HRP_Clusterin_Antibody_1_032118
Western blot of HRP anti-Clusterin antibody (clone A15113A). Lane 1: Molecular weight marker; Lane2: 50 ng of recombinant human Clusterin (cat. No. 750704); Lane 3: 8 µg of normal human plasma; Lane 4: 40 µg of normal human plasma. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A15113A_HRP_Clusterin_Antibody_1_032118
    Western blot of HRP anti-Clusterin antibody (clone A15113A). Lane 1: Molecular weight marker; Lane2: 50 ng of recombinant human Clusterin (cat. No. 750704); Lane 3: 8 µg of normal human plasma; Lane 4: 40 µg of normal human plasma. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A15113A_HRP_Clusterin_Antibody_2_032118
    IHC staining of HRP anti-Clusterin antibody (clone A15113A) on formalin-fixed paraffin-embedded normal (left panel) and Alzheimer’s disease (right panel) human brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissues were incubated with 5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm.
Cat # Size Price Quantity Avail. Save
848703 25 µg $115
Check Availability


Need larger quantities of this item?
Request Bulk Quote
848704 100 µg $225
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Clusterin, also known as Apolipoprotein J, is a heterodimeric glycoprotein that is a member of the heat-shock protein family. The protein exhibits a wide array of biological functions including clearance of cellular debris through chaperone activity, apoptosis, lipid transportation, cell clustering, and complement inhibition. Clusterin is involved in many diseases related to oxidative stress and aging, including neurodegenerative disorders, cancers, diabetes, and inflammatory diseases.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human clusterin.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 – 1.0 µg per ml. For immunohistochemistry, a concentration range of 1.0 – 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2728569 (BioLegend Cat. No. 848703)
AB_2728570 (BioLegend Cat. No. 848704)

Antigen Details

Structure
Clusterin is a protein of 449 amino acids with a molecular mass of 52 kD.
Distribution

Tissue distribution: Ubiquitously expressed.
Cellular distribution: Primarily extracellular in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Intracellular clusterin is primarily present in the endoplasmic reticulum, cytosol, mitochondrion, and nucleus.

Function
Clusterin is involved in several biological processes including extra- and intracellular chaperone activity, pro- and anti-apoptotic properties, lipid transportation, cell clustering, modulation of NF-kappa-B transcriptional activity, and complement inhibition.
Interaction
HDL
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Complement, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Apolipoproteins
Antigen References

1. Trougakos IP. 2013. Gerontology 59:514. PubMed
2. Rizzi F, Bettuzzi S. 2010. Endocr. Relat. Cancer 17:R1. PubMed
3. Desikan RS, et al. 2014. JAMA Neurol. 71:180. PubMed
4. Carver JA, et al. 2003. IUBMB Life. 55:661. PubMed

Gene ID
1191 View all products for this Gene ID
UniProt
View information about Clusterin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/26/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account