HRP anti-Clathrin Heavy Chain (CLTCL2) Antibody

Pricing & Availability
Clone
TD.1 (See other available formats)
Other Names
Clathrin heavy chain 1, clathrin, heavy polypeptide-like 2, clathrin heavy chain on chromosome 17
Isotype
Mouse IgG1
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Product Citations
publications
TD-1_HRP_Clathrin_Heavy_Chain_Antibody_WB_082718
Western blot of HRP anti-Clathrin Heavy Chain (CLTCL2) antibody (clone TD.1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • TD-1_HRP_Clathrin_Heavy_Chain_Antibody_WB_082718
    Western blot of HRP anti-Clathrin Heavy Chain (CLTCL2) antibody (clone TD.1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
Cat # Size Price Quantity Avail. Save
813903 25 µg $125
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813904 100 µg $275
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Description

Clathrin consists of three heavy chains (192 kD) and three light chains (LCa or LCb, 25-29 kD) and self assembles into a polyhedral cytoskeletal element. The self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised using the globular N-terminal domain of clathrin heavy chain.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody reacts with clathrin heavy chains (CHC). The antibody is useful for immunoblotting of clathrin heavy chains from cow CHC and immunofluorescence. It does not react with native clathrin in immunoprecipitation.

Reactivity to Drosophila was only validated with the purified format.

Application References

(PubMed link indicates BioLegend citation)
  1. Hood FE and Royle SJ. 2009. J Cell Sci. 122:2185-2190. (ICC) PubMed
  2. Jiang R, et al. 2000. J Biol Chem 275(12):8439-8447. (IP)
RRID
AB_2750414 (BioLegend Cat. No. 813903)
AB_2750415 (BioLegend Cat. No. 813904)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID
1213 View all products for this Gene ID
UniProt
View information about Clathrin Heavy Chain (CLTC) on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08/27/2018

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