- A17035K (See other available formats)
- Other Names
- Beta-Secretase 1, Membrane-Associated Aspartic Protease 2, Beta-Site APP Cleaving Enzyme 1, Aspartyl Protease 2, Memapsin-2, BACE, ASP2, Transmembrane Aspartic Proteinase Asp2, Beta-Secretase 1 Precursor Variant 1, Beta-Site APP-Cleaving Enzyme 1
- Mouse IgG2b, κ
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Beta-site APP-cleaving enzyme 1 (BACE1), also known as aspartyl protease 2 (ASP-2) and memapsin 2, is a type I transmembrane aspartic protease with 501 amino acid that is related to the pepsin family and the retroviral aspartic proteases. The BACE1 catalytic domain contains aspartic protease active site motifs at amino acids 93-96 and 289-292, with each motif containing the highly conserved sequence defining aspartic proteases, D(T/S)G(T/S). BACE1 is expressed at low levels in most cell types of the body, although it is more highly expressed in neurons. In the brain, the protein is highly expressed in the substantia nigra, locus coruleus, and medulla oblongata. It is localized within acidic intracellular compartments including endosomes and trans-Golgi network (TGN). In the brain, BACE1 is involved in proteolytic processing of the amyloid precursor protein (APP) which generates the b amyloid peptide- a peptide that is associated with Alzheimer’s disease. BACE1 cleaves APP at the N-terminus of the b amyloid peptide sequence, between residues 671 and 672, which leads to extracellular release of b-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by g-secretase. The physiological function of BACE1 is not clearly understood, although it may modulate sodium currents by cleavage of voltage-gated Na+ channel accessory subunits (Navβ1-4). Also, BACE1 has been implicated in the regulation of myelination and myelin sheath thickness and in the control of motor coordination via cleavage of neuregulin-1 and neuregulin-3, and also in axon guidance via cleavage of close homolog of L1 (CHL1).Product Details
- Antibody Type
- Host Species
- Recombinant human BACE1 protein.
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
AB_2783427 (BioLegend Cat. No. 852903)
AB_2783428 (BioLegend Cat. No. 852904)
- Human BACE1 is a 501 amino acid protein with a molecular mass of 56 kD and exists as 4 isoforms.
Tissue sources: Ubiquitously expressed. Highest levels in the brain and pancreas.
Cellular distribution: Plasma membrane, endoplasmic reticulum, and golgi apparatus.
- BACE1 is involved in proteolytic processing of the amyloid precursor protein (APP). It cleaves APP at the N-terminus of the amyloid β peptide sequence, between residues 671 and 672 of APP.
- APP, IL1-R2, PSGL-1, ST6Gal-1, GGA1, GGA2, GGA3, RTN3, RTN4, SNX6, PCSK9, NAT8 and NAT8B.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Enzymes and Regulators, Secretases
- Antigen References
- Vassar R, et al. 2014. J. Neurochem. 130:4-28.
- Kandalepas PC, Vassar R. 2014. Curr. Alzheimers Res. 11:441-9.
- Kandalepas PC, Vassar R. 2012. J. Neurochem. 120(Suppl. 1):55-61.
- Gruninger-Leitch F, et al. 2002. J. Biol. Chem. 277:4687-93.
- Koelsch G. 2017. Molecules. 22(10): E1723.
- Gene ID
- 23621 View all products for this Gene ID
- View information about BACE1 on UniProt.org
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