HRP anti-ATF6β Antibody

Pricing & Availability
Clone
W17035A (See other available formats)
Other Names
Cyclic AMP-dependent transcription factor ATF-6 beta, ATF-6 beta, Activating transcription factor 6 beta, ATF6-beta
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17035A_HRP_ATF6-beta_Antibody_1_090418
Western blot of HRP anti-ATF6β antibody (clone W17035A). Lane 1: Molecular weight marker; Lane 2: 20 µg of SH-SY5Y cell lysate; Lane 3: 20 µg of Hela lysate. The blot was incubated with 0.05 µg/ml of the primary antibody for 1 hour at room temperature. Enhanced chemiluminescence was used as the detection system.
  • W17035A_HRP_ATF6-beta_Antibody_1_090418
    Western blot of HRP anti-ATF6β antibody (clone W17035A). Lane 1: Molecular weight marker; Lane 2: 20 µg of SH-SY5Y cell lysate; Lane 3: 20 µg of Hela lysate. The blot was incubated with 0.05 µg/ml of the primary antibody for 1 hour at room temperature. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
853203 25 µg $125
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853204 100 µg $265
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Description

ATF6 encodes a transcription factor that is anchored in the endoplasmic reticulum (ER) and activated during the unfolded protein response (UPR) to protect cells from ER stress. Under conditions of ER stress, ATF6 is transported from the ER to the Golgi apparatus, where it is cleaved by Golgi-resident proteases to release the cytosolic DNA-binding portion. Then the processed ATF6 moves to the nucleus to activate gene expression. Deletion of the isoform activating transcription factor 6α (ATF6α) and its paralog ATF6β results in embryonic lethality and notochord dysgenesis in nonhuman vertebrates, and loss-of-function mutations in ATF6α are associated with malformed neuroretina and congenital vision loss in humans. Altered ATF6 function in neurodegenerative disorders such as  Parkinson's disease has been observed. 

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human ATF6β recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.05 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2750114 (BioLegend Cat. No. 853203)
AB_2750115 (BioLegend Cat. No. 853204)

Antigen Details

Structure
ATF6β is a 703 amino acid protein with a molecular weight mass of ~80 kD.
Distribution

Tissue distribution: ATF6β is ubiquitously expressed in numerous cell types including cells in of the central nervous system (CNS).

Cellular distribution: Cytoplasmic, nucleus, ER, and golgi apparatus.

Function
ATF6β is a transmembrane glycoprotein that functions as a transcription activator and initiates the unfolded protein response during ER stress.
Interaction
ATF6β interacts with ER chaperone immunoglobulin-binding protein (BiP), and can be cleaved by lumenal site 1 protease (S1P) and intra-membrane site 2 protease (S2P).
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Transcription Factors
Molecular Family
Endoplasmic Reticulum Markers
Antigen References
  1. Shen J, et al. 2002. Dev Cell. 3:99-111
  2. Ron D and Walter P. 2007. Nat Rev Mol Cell Biol. 8: 519-529
  3. Credle JJ, et al. 2015. Neurobiol Dis. 76: 112-125
  4. Jin JK, et al. 2017. Circ Res. 120: 862-875
  5. Kroeger H, et al. 2018. Sci Signal. 11: 5785
Gene ID
1388 View all products for this Gene ID
UniProt
View information about ATF6beta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/05/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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