HRP anti-Ankyrin-B Antibody

Pricing & Availability
Clone
N105/17 (See other available formats)
Other Names
Ankyrin-2, ankyrin B, ankyrin, brain, non-erythroid ankyrin, ankyrin-2 nonerythrocytic
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
1_N105slash17__HRP_Ankyrin-B_Antibody_WB_050918
Western blot of HRP anti-Ankyrin-B antibody (clone N105/17/30). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody. HRP anti-β-actin antibody was used as loading control. Enhanced chemiluminescence was used as the detection system. Multiple bands observed by WB represent multiple splice variants and endogenous proteolysis of Ankyrin B.
  • 1_N105slash17__HRP_Ankyrin-B_Antibody_WB_050918
    Western blot of HRP anti-Ankyrin-B antibody (clone N105/17/30). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody. HRP anti-β-actin antibody was used as loading control. Enhanced chemiluminescence was used as the detection system. Multiple bands observed by WB represent multiple splice variants and endogenous proteolysis of Ankyrin B.
  • N105slash17_HRP_Ankyrin_Antibody_1_050918
    IHC staining of HRP anti-Ankyrin-B antibody (clone N105/17) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N105slash17_HRP_Ankyrin_Antibody_2_050918
    IHC staining of HRP anti-Ankyrin-B antibody (clone N105/17) on formalin-fixed paraffin-embedded mouse hippocampal tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N105slash17_HRP_Ankyrin_Antibody_3_050918
    IHC staining of HRP anti-Ankyrin-B antibody (clone N105/17) on formalin-fixed paraffin-embedded rat hippocampal tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
821503 25 µg $100
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821504 100 µg $170
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Description

Ankyrins are adaptor proteins that mediate the attachment of membrane proteins to the cytoskeleton. Ankyrin-B (Ankyrin-2) is highly expressed in cardiac tissue where it has an essential role in stabilizing ion transporters and channels in cardiomyocytes in the membrane. Mutations in ankyrin-B are associated with cardiac arrhythmia and ankyrin-B may be involved in human heart failure.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 203-496 (ankyrin repeats 6 to 14) of human Ankyrin-B.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry, a concentration range of 1 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2734586 (BioLegend Cat. No. 821503)
AB_2734587 (BioLegend Cat. No. 821504)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
287 View all products for this Gene ID
UniProt
View information about Ankyrin-B on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/09/2018

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