HRP anti-AMIGO-1 Antibody

Pricing & Availability
Clone
L86A/37 (See other available formats)
Other Names
Amphoterin-induced protein 1, alivin-2, amphoterin-induced gene and ORF
Isotype
Mouse IgG2b
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Product Citations
publications
1_L86Aslash37_HRP_AMIGO-1_IHCP_Antibody_1_061518
IHC staining of HRP anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1_L86Aslash37_HRP_AMIGO-1_IHCP_Antibody_1_061518
    IHC staining of HRP anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2_L86Aslash37_HRP_AMIGO-1_IHCP_Antibody_2_061518
    IHC staining of HRP anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_L86Aslash37_HRP_AMIGO-1_Antibody_WB_061518
    Western blot of HRP anti-AMIGO-1 antibody (clone L86A/37). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C. HRP anti-β-tubulin antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
822103 25 µg $100
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822104 100 µg $170
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Description

Amphoterin-induced protein 1 (AMIGO-1) is a transmembrane protein containing six LRR domains and one Ig domain and is highly expressed in the mammalian nervous system. It promotes growth and fasciculation of neurites from cultured hippocampal neurons and may be involved in fasciculation as well as myelination of developing neural axons. AMIGO-1 may have a role in regeneration as well as neural plasticity in the adult nervous system.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 395-493 (cytoplasmic C-terminus) of human AMIGO-1.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

IHC-P - Validated
WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml.It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

The reactivity of HRP anti-AMIGO-1 antibody was validated in FFPE mouse and rat brain tissues.

Application References

(PubMed link indicates BioLegend citation)
  1. Mandikian D, et al. 2014. Comp Neurol. 522(15): 3555. (IHC-P)
RRID
AB_2734589 (BioLegend Cat. No. 822103)
AB_2734590 (BioLegend Cat. No. 822104)

Antigen Details

Structure
Amigo-1 has an apparent molecular weight ranging from 60-80 kD.
Distribution

Tissue distribution: Nervous system<br>Cellular distribution: Plasma membrane

Biology Area
Cell Biology, Neuroscience, Synaptic Biology, Transcription Factors
Molecular Family
Adhesion Molecules
Antigen References

1. Chen Y, et al. 2006. Brain Res Rev. 51(2):265.

Gene ID
57463 View all products for this Gene ID
UniProt
View information about AMIGO-1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/20/2018

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