HRP anti-AMIGO-1 Antibody

Pricing & Availability
Clone
L86/36 (See other available formats)
Other Names
Amphoterin-induced protein 1, alivin-2, amphoterin-induced gene and ORF, Ali2, AMIGO and KIAA1163
Isotype
Mouse IgG1
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Product Citations
publications
L86-36_HRP_AMIGO-1_Antibody_1_040918
Western blot of HRP anti-AMIGO-1 antibody (clone L86/36). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 10 µg/mL of clone L86/36 or mouse IgG overnight at 4°C. HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • L86-36_HRP_AMIGO-1_Antibody_1_040918
    Western blot of HRP anti-AMIGO-1 antibody (clone L86/36). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 10 µg/mL of clone L86/36 or mouse IgG overnight at 4°C. HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • L86-36_HRP_AMIGO-1_Antibody_2_040918
    IHC staining of HRP anti-AMIGO-1 antibody (clone L86/36) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • L86-36_HRP_AMIGO-1_Antibody_3_040918
    IHC staining of HRP anti-AMIGO-1 antibody (clone L86/36) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
804803 25 µg $100
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804804 100 µg $170
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Description

Amphoterin-induced protein 1 (AMIGO-1) is a transmembrane protein containing six LRR domains and one Ig domain and is highly expressed in the mammalian nervous system. It promotes growth and fasciculation of neurites from cultured hippocampal neurons and may be involved in fasciculation as well as myelination of developing neural axons. AMIGO-1 may have a role in regeneration as well as neural plasticity in the adult nervous system.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 395-493 (cytoplasmic C-terminus) of human AMIGO-1.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC), and immunohistochemistry (IHC).

This antibody does not cross-react with Amigo-2 or Amigo-3.

RRID
AB_2728528 (BioLegend Cat. No. 804803)
AB_2728529 (BioLegend Cat. No. 804804)

Antigen Details

Structure
*Expected MW: 60 - 80 kD (depending on maturity & glycosylation)
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Gene ID
57463 View all products for this Gene ID
UniProt
View information about AMIGO-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/10/2018

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