HRP anti-α-Synuclein, aggregated Antibody

Pricing & Availability
Clone
Syn-O2 (See other available formats)
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP)
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Product Citations
publications
Syn-O2_HRP_a-Synuclein_Antibody_041918
IHC staining of HRP anti-α-Synuclein, aggregated antibody (clone Syn-O2) on formalin-fixed paraffin-embedded normal (left panel) and Parkinson’s disease (right panel) human brain tissues. Following antigen retrieval using 88% formic acid, the tissues were incubated with 1.0 µg/mL of the primary antibody for 1 hour at room temperature. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • Syn-O2_HRP_a-Synuclein_Antibody_041918
    IHC staining of HRP anti-α-Synuclein, aggregated antibody (clone Syn-O2) on formalin-fixed paraffin-embedded normal (left panel) and Parkinson’s disease (right panel) human brain tissues. Following antigen retrieval using 88% formic acid, the tissues were incubated with 1.0 µg/mL of the primary antibody for 1 hour at room temperature. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
847603 25 µg $105
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847604 100 µg $235
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Description

α-synuclein is expressed principally in the nervous system, but it is also produced in the other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in the presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in the nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Aggregated α-synuclein
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not cross-react with β- or γ-synucleins using Dot Blot as detection method.

Application References

(PubMed link indicates BioLegend citation)
  1. Majbour NK, et al. 2016. Mol. Neurodegener. 11:7. (IHC, ELISA Capture, Dot Blot)
  2. Vaikath NN, et al. 2015. Neurobiol Dis. 79:81. (IHC, ELISA Capture, Dot Blot)
RRID
AB_2734620 (BioLegend Cat. No. 847603)
AB_2734621 (BioLegend Cat. No. 847604)

Antigen Details

Structure
α-synuclein's canonical isoform consists of 140 amino acids, which consists of four 11-residue repeats containing the consensus sequence KTKEGV. While, α-synuclei has an apparent molecular mass of 14 kD.
Distribution

Cellular distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extraceullar.
Tissue distribution: Primarily in the nervous system, but is lower expressed in other tissues.

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein
Antigen References

1. Mor DE, et al. 2016. Neurobiol. Dis. 88:66.
2. Jucker M, Walker LC. 2013. Nature. 501(7465):45.
3. Bartels T, et al. 2011. Nature. 477(7362):107.
4. Devine MJ, et al. 2011. Mov. Disord. 26:2160.

Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein, aggregated on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/20/2018

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