HRP anti-α-Synuclein, 103-108 Antibody

Pricing & Availability
Clone
4B12/Synuclein (See other available formats)
Other Names
NACP, PARK1, PARK4, PD1, Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-A beta component of AD amyloid Parkinson disease
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_4B12-Synuclein_HRP_alpha-Synuclein_Antibody_WB_072417
Western blot of HRP anti-α-Synuclein, 103-108 (clone 4B12/Synuclein). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • 1_4B12-Synuclein_HRP_alpha-Synuclein_Antibody_WB_072417
    Western blot of HRP anti-α-Synuclein, 103-108 (clone 4B12/Synuclein). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • 2_4B12Synuclein_HRP_alpha-Synuclein_Antibody_IHC_011018
    IHC staining of HRP anti-α-Synuclein, 103-108 (clone 4B12/Synuclein) on formalin-fixed paraffin-embedded normal human (left) and Parkinson’s disease (right) brain tissue. After antigen retrieval using 70% formic acid, the tissues were incubated with 2.5 µg/mL of the primary antibody at for 24 hours at 4°C. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided.
Cat # Size Price Quantity Avail. Save
807805 25 µg $110
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807806 100 µg $270
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Description

α-Synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including  the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are a-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-Synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using a purified, E. coli produced human α-synuclein.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody reacts with human α-synuclein. It does not react with mouse or rat α-synuclein. The epitope lies within amino acids 103-108 of α-synuclein.

Additional reported applications (for the relevant formats) include: ELISA2, Western blotting1,2,3,4,6,8, and immunoprecipitation4.

Application References

(PubMed link indicates BioLegend citation)
  1. Lahut S, et al. 2017. Dis Model Mech(WB) PubMed
  2. Landeck N, et al. 2016. Mol Neurodegener. 11(1): 61. (ELISA, WB) PubMed
  3. Moussaud S, et al. 2015. Expert Opin Ther Targets. 19(5): 589. (WB) PubMed
  4. Helferich AM., et al. 2015. Mol Neurodegener. 10(1): 66. (IHC, IP, WB) PubMed
  5. Bodis‐Wollner I, et al. 2014. Ann Neurol. 75(6): 964. (IHC)
  6. Shen W, et al. 2014. J Neurochem. 129(5): 884. (WB)
  7. Fagerqvist T, et al. 2013. J Neurochem. 126(1): 131. (IHC)
  8. Ebrahimi-Fakhari D, et al. 2011. J Neurosci. 31(41): 14508. (WB) PubMed
  9. Welander H, et al. 2011. Biochem Biophys Res Commun. 412(1): 32. (IHC)
RRID
AB_2721331 (BioLegend Cat. No. 807805)
AB_2721332 (BioLegend Cat. No. 807806)

Antigen Details

Structure
α-Synuclein’s canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Distribution

Tissue distribution: Primarily nervous system, but lower expression in other tissues.
Cellular distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein
Antigen References
  1. Lee JM, et al. 2017. J Neuropathol Exp Neurol. 76(1):2.
  2. Kang SS, et al. 2017. Proc Natl Acad Sci. 114(5): 1183.
  3. Valdes P, et al. 2016. Front Neuroanat. 10:123.
  4. Emamzadeh FN. 2016. J Res Med Sci. 9(21):29.
Gene ID
6622 View all products for this Gene ID
UniProt
View information about α-Synuclein, 103-108 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12/14/2017

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