Helix NP™ Green

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Other Names
Sytox™ Green
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Helix_NP_Green_1_FC_112916
One-day-old C57BL/6 mouse splenocytes were stained with 5 µM Helix NP™ Green and APC Annexin V with no wash.
  • Helix_NP_Green_1_FC_112916
    One-day-old C57BL/6 mouse splenocytes were stained with 5 µM Helix NP™ Green and APC Annexin V with no wash.
  • Helix_NP_Green_2_IF_112916
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/mL of Alexa Fluor® 647 anti-Cytokeratin (pan reactive) antibody (red) in blocking buffer overnight followed by 1:500 dilution of Helix NP™ Green (green) and Flash Phalloidin™ Red 594 (blue) staining for 15 minutes at room temperature. The image was captured with a 60X objective.
  • Helix_NP_Green_3_IHCF_112916
    Mouse frozen cerebellum tissue was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for one hour. Then the tissue was intracellularly stained with 1:500 dilution of Helix NP™ Green (green) for 15 minutes at room temperature. The image was captured with a 20X objective.
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425303 250 µl $180
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Description

Helix NP™ Green is a green-emitting nucleic acid stain. It is impermeant to live cells and thus can be used for the discrimination of live and dead cells. In immunofluorescence microscopy, it can be used as a nuclear counterstain in cells and tissue. It is optimally excited at 495 nm with an emission at 519 nm, which can be detected in the Alexa Fluor® 488 or FITC channel.

Product Details
Technical data sheet

Product Details

Preparation
Helix NP™ Green is supplied at 250 µl per vial.
Concentration
5.0 mM
Storage & Handling
Upon receipt, store at -20°C.
Application

FC - Quality tested
IF, IHC-F - Validated

Recommended Usage

For use in viability assays, the optimal concentration can range from 1nM -10 nM. For immunofluorescence microscopy and immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 0.5 μM -10 μM. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Helix NP™ Green is a cell-impermeant nucleic acid probe suitable for use as a viability dye in flow cytometry.  It can also be used for viability in microscopy on live cells or as a nuclear counterstain on fixed and permeabilized cells and tissues. It is a green-emitting dye with an excitation/emission max of 495 nm/519 nm that can be detected in the Alexa Fluor® 488 or FITC channel. 

Protocol for viability staining using Helix NP™ Green:

1. Isolate cells following protocol of choice.
2. Dilute Helix NP™ Green to required concentration. We recommend titrating the reagent to determine optimal concentration for cells of interest. We have observed good results in flow cytometry at 1-10 nM.
3. Following the addition of Helix NP™ Green to cells, do not wash, cells are ready for acquisition.
4. Analyze cells on a cytometer equipped with the 488nm blue laser.

Antigen Details

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
Gene ID
NA

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Go To Top Version: 2    Revision Date: 12/02/2016

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