GoInVivo™ Purified anti-mouse CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
10F.9G2 (See other available formats)
Other Names
B7-H1, PD-L1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
10F.9G2_GoInVivoPurified_CD274_B7H1_PDL1_Antibody_FC_010716
Anti-mouse PD-L1 inhibits the binding of PD-1 to immobilized PD-L1. Immobilized recombinant PD-L1-Fc chimera (coated at 1 µg/mL) was pre-incubated with increasing concentrations of anti-mouse PD-L1 (clone 10F.9G2, purple circles) or isotype control (clone RTK4530, purple squares), followed by incubation with a fixed concentration of PD-1-Fc chimera (1 µg/mL). Clone 10F.9G2 inhibits PD-1/PD-L1 interaction in a dose dependent manner.
  • 10F.9G2_GoInVivoPurified_CD274_B7H1_PDL1_Antibody_FC_010716
    Anti-mouse PD-L1 inhibits the binding of PD-1 to immobilized PD-L1. Immobilized recombinant PD-L1-Fc chimera (coated at 1 µg/mL) was pre-incubated with increasing concentrations of anti-mouse PD-L1 (clone 10F.9G2, purple circles) or isotype control (clone RTK4530, purple squares), followed by incubation with a fixed concentration of PD-1-Fc chimera (1 µg/mL). Clone 10F.9G2 inhibits PD-1/PD-L1 interaction in a dose dependent manner.
Cat # Size Price Quantity Avail. Save
124328 5 mg $700
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124329 25 mg $2,150
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124330 50 mg $3,250
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124325 100 mg $4,850
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124326 500 mg $16,750
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124327 1 g $26,000
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Description

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <1.0 EU/mg of the protein. (<0.1 pg/µg of the protein) as determined by the LAL test.
Preparation
The GoInVivo™ antibody was purified by affinity chromatography. The antibody is bottled at the concentration indicated on the vial, typically between 1 mg/ml and 3 mg/ml.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IF, Block - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage

Additional reported applications (for the relevant formats) include: immunofluorescence4 and blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections11, 12.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Maier H, et al. 2007. J. Immunol. 178:2714.
  2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
  3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
  4. Grabie N, et al. 2007. Circulation 116:2062. (IF)
  5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
  6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
  7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
  8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
  9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
  10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
  12. Grabie N, et al. 2007. Circulation 116:2062-71. (IHC)
  13. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
Product Citations
  1. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  2. Wang C, et al. 2018. Sci Transl Med. 10. PubMed
Publication Library
RRID
AB_2566281 (BioLegend Cat. No. 124328)
AB_2566282 (BioLegend Cat. No. 124329)
AB_2566283 (BioLegend Cat. No. 124330)
AB_2566092 (BioLegend Cat. No. 124325)
AB_2566093 (BioLegend Cat. No. 124326)
AB_2566094 (BioLegend Cat. No. 124327)

Antigen Details

Structure
40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.

Gene ID
60533 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 11/30/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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