- A15158B (See other available formats)
- Other Names
- Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
- Mouse IgG1, κ
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STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Two isoforms of STAT1, with apparent molecular weights of 88 and 91 kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Human STAT1 peptide phosphorylated at Ser 727.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ChIP - Quality tested
WB, ICFC, ICC - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg/ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
AB_2687272 (BioLegend Cat. No. 686413)
AB_2687273 (BioLegend Cat. No. 686414)
- 750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization.
Translocates to the nucleus when phosphorylated.
- Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.
- Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Nuclear Markers
- Antigen References
1. Durbin JE, et al. 1996. Cell. 84:443.
2. Darnell JE Jr, et al. 1994. Science 264:1415.
3. Chen X, et al. 1998. Cell. 93:827.
4. Ramana CV, et al. 2000. Oncogene. 19:2619.
- Gene ID
- 6772 View all products for this Gene ID
- View information about STAT1 Phospho on UniProt.org
- BioLegend’s Tools for Chromatin Immunoprecipitation (ChIP) Assays - Video
- Chromatin Immunoprecipitation (ChIP) Assay Protocol
- Immunocytochemistry Staining Protocol
- Western Blotting Protocol
- Intracellular Staining With True-Phos™ Perm Buffer in Cell Suspensions Protocol
- Intracellular Staining With True-Phos™ Perm Buffer in Whole Blood
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