- CTD4H8 (See other available formats)
- Other Names
- DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit
- Mouse IgG1
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RPB1 is the catalytic and largest component of RNA polymerase II, which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with RPB2, the second largest subunit. Polymerase II (Pol II) is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relatively to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft, and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single DNA template strand of the promoter is positioned within the central active site cleft of Pol II. Then, a bridging helix emanates from RPB1 and crosses the cleft near the catalytic site, which acts as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations during each neuocleotide addition. This promotes translocation of Pol II. Pol II moves on the template during transcription elongation. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II's largest subunit (RPB1), which serves as a platform for assembling factors that regulate transcription initiation, elongation, termination, and mRNA processing. It can act as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, being able to conform as both a replicate and transcriptase for the viral RNA circular genome.Product Details
- Human, Mouse, Rat, Drosophilia, Yeast (S. cerevisiae), Zebrafish
- Antibody Type
- Host Species
- The immunogen used was a peptide containing 10 repeats of the synthetic peptide YSPTSPS using chemically synthesized phospho-ser5.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ChIP - Quality tested
WB - Verified
ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:300-1:500 by volume. For Western blotting the suggested working dilution is 1:500 by volume. For immunofluorescent staining, the suggested dilution is 1:500 by volume.
It is recommended that the reagent to be titrated for optimal performance before each experiment.
- Application Notes
This antibody is effective in immunoblotting, immunoprecipitation and ELISA. It can also be used in chromatin immunoprecipitation assays.
The antibody CTD4H8 recognizes the C-terminal repeat of the largest subunit of RNA polymerase II from HeLa and S. cerevisiae cells. This antibody recognizes both the phosphorylated and unphosphorylated forms of the RNA polymerase II.
Based on sequence identify, this clone is liable to react with a broad range of species.
Clone CTD4H8 is also known as 4H8 or CTD 4H8.
Predicted MW is ~ 217 kD, Observed MW is on WB gel is ~240 kD
(PubMed link indicates BioLegend citation)
- Kristjuhan A, et al. 2002. Mol. Cell. 10:925.
- Charos AE, et al. 2012. Genome Res. 22:1668. (ChIP)
- Rafalska-Metcalf IU, et al. 2010. PLoS One 5:e10272. (ICC) PubMed
AB_2715775 (BioLegend Cat. No. 904003)
AB_2715774 (BioLegend Cat. No. 904004)
- Biology Area
- Cell Biology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID
- 5430 View all products for this Gene ID
- View information about RNA Polymerase II on UniProt.org
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