GMP FITC anti-human CD4 Antibody, CD4, SK3

Pricing & Availability

Clone: SK3 (See other available formats)
Other Names: T4, Leu3a
Isotype: Mouse IgG1, κ
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Product Citations: publications

SK3_GMP_FITC_CD4_Antibody_051722 — Typical results from human peripheral blood lymphocytes stained either with SK3 FITC used at 5 µL/test (filled histogram) or with isotype control (open histogram).

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260104

100 tests

$235

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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details

Technical data sheet

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.

Concentration

50 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)

Application References (PubMed link indicates BioLegend citation)

Evans RL, et al. 1981. Immunol. 78:544
Arno A et al. 1999. J. Infect. Dis. 180:56
Muech M, et al. 1997. Blood 89:1364
Wang L, et al. 2012. Cytometry A. 81:567. PubMed

RRID

AB_2922514 (BioLegend Cat. No. 260104)

Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

Batch-to-batch consistency
Material traceability
Documented procedures
Documented employee training
Equipment maintenance and monitoring records
Lot-specific certificates of analysis
Quality audits per ISO 13485:2016
QA review of released products

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D et al. 1996. Immunol. Today 17:476.
2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD4 Reagents Request Custom Conjugation

Description	Clone	Applications
PerCP anti-human CD4	SK3	FC
Purified anti-human CD4	SK3	FC,IP
FITC anti-human CD4	SK3	FC
PE anti-human CD4	SK3	FC
PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Biotin anti-human CD4	SK3	FC
PE/Cyanine7 anti-human CD4	SK3	FC
APC anti-human CD4	SK3	FC
APC/Cyanine7 anti-human CD4	SK3	FC
Alexa Fluor® 488 anti-human CD4	SK3	FC
Pacific Blue™ anti-human CD4	SK3	FC
Alexa Fluor® 700 anti-human CD4	SK3	FC
Purified anti-human CD4 (Maxpar® Ready)	SK3	FC,CyTOF®
PE anti-human CD4	SK3	FC
Brilliant Violet 421™ anti-human CD4	SK3	FC
Brilliant Violet 510™ anti-human CD4	SK3	FC
Alexa Fluor® 647 anti-human CD4	SK3	FC
FITC anti-human CD4	SK3	FC
APC/Fire™ 750 anti-human CD4	SK3	FC
PE/Dazzle™ 594 anti-human CD4	SK3	FC
Pacific Blue™ anti-human CD4	SK3	FC
Brilliant Violet 785™ anti-human CD4	SK3	FC
Brilliant Violet 750™ anti-human CD4	SK3	FC
Brilliant Violet 605™ anti-human CD4	SK3	FC
Brilliant Violet 711™ anti-human CD4	SK3	FC
PE/Cyanine7 anti-human CD4	SK3	FC
TotalSeq™-A0045 anti-human CD4	SK3	PG
TotalSeq™-C0045 anti-human CD4	SK3	PG
PE/Cyanine5 anti-human CD4	SK3	FC
PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Spark Blue™ 550 anti-human CD4	SK3	FC
Spark NIR™ 685 anti-human CD4	SK3	FC
APC anti-human CD4	SK3	FC
KIRAVIA Blue 520™ anti-human CD4	SK3	FC
APC/Fire™ 810 anti-human CD4	SK3	FC
PE/Fire™ 640 anti-human CD4	SK3	FC
PE/Fire™ 700 anti-human CD4	SK3	FC
PerCP anti-human CD4	SK3	FC
Spark Violet™ 538 anti-human CD4 Antibody	SK3	FC
Spark YG™ 581 anti-human CD4	SK3	FC
Alexa Fluor® 660 anti-human CD4	SK3	FC
Spark YG™ 593 anti-human CD4	SK3	FC
PE/Fire™ 810 anti-human CD4 Antibody	SK3	FC
APC/Fire™ 750 anti-human CD4	SK3	FC
Spark Blue™ 574 anti-human CD4	SK3	FC
GMP APC anti-human CD4	SK3	FC
TotalSeq™-B0045 anti-human CD4 Antibody	SK3	PG
Spark Violet™ 423 anti-human CD4 Antibody	SK3	FC
GMP Pacific Blue™ anti-human CD4	SK3	FC
Spark UV™ 387 anti-human CD4	SK3	FC
GMP PE anti-human CD4	SK3	FC
GMP FITC anti-human CD4	SK3	FC
Spark Red™ 718 anti-human CD4	SK3	FC
GMP PerCP anti-human CD4	SK3	FC
GMP PE/Cyanine7 anti-human CD4	SK3	FC
Spark Violet™ 500 anti-human CD4	SK3	FC
GMP PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Brilliant Violet 650™ anti-human CD4	SK3	FC
PerCP/Fire™ 806 anti-human CD4	SK3	FC
Spark Blue™ 515 anti-human CD4	SK3	FC
GMP APC/Fire™ 750 anti-human CD4	SK3	FC
Spark Violet™ 423 anti-human CD4	SK3	FC
PerCP/Fire™ 780 anti-human CD4	SK3	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/01/2022

For research use only. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.