FITC anti-mouse/rat CD29 Antibody

Pricing & Availability
Clone
HMβ1-1 (See other available formats)
Other Names
integrin β1, VLA-β chain, β1 integrin, GPIIa, ITGB1
Isotype
Armenian Hamster IgG
Ave. Rating
4 reviews
Product Citations
publications
Hmβ1-1
Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
  • Hmβ1-1
    Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
  • HMβ1-1_mouse
    Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
See FITC spectral data
Cat # Size Price Quantity Avail. Save
102205 50 µg $85
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102206 500 µg $280
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Description

CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMß1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Purified mouse VLA-4 (α4β1, CD49d/CD29)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry4 of acetone-fixed frozen sections, in vitro blocking of the adhesion of mouse tumor cell lines to extracellular matrix proteins and in vitro inhibition of T cell proliferative responses1, and in vivo inhibition of neutrophil migration2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102210).

Application References

(PubMed link indicates BioLegend citation)
  1. Noto K, et al. 1995. Int. Immunol. 7:835.
  2. Ridger VC, et al. 2001. J. Immunol. 166:3484.
  3. Jia W, et al. 2005. Blood 106:3854.PubMed
  4. Economopoulou M, et al. 2005. Blood 106:3831.
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  6. Eisenmann KM, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M703243200.PubMed
  7. Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed
  8. Kim DT, et al. 2008. Blood 111:2929.PubMed
  9. Hayashi Y, et al. 2008. J Pharmacol Exp Ther. 326:523. PubMed
  10. Carlson TR, et al. 2008. Development. 135:2193. PubMed
  11. Sangaletti S, et al. 2008. Cancer Res. 68:9050. (Block) PubMed
  12. Baker CM, et al. 2012. PNAS. PubMed.
  13. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
Product Citations
  1. Chen K, et al. 2009. Proc Natl Acad Sci U S A. 106:17413. PubMed
  2. Gu B, et al. 2009. J Cell Biol. 185:811. PubMed
  3. Rey-Barroso J, et al. 2013. Cell Signal. 25:848. PubMed
  4. Cai W, et al. 2013. J Cell Sci. 126:2877. PubMed
  5. Contador-Troca M, et al. 2013. Carcinogenesis. 34:2683. PubMed
  6. Takahashi M, et al. 2009. Am J Physiol Heart Circ Physiol. 298:415. PubMed
  7. Watzlawik J, et al. 2010. Glia. 58:1782. PubMed
  8. Zou M, et al. 2014. J Biol Chem. 289:17620. PubMed
  9. Tong L, et al. 2016. Sci Rep. 6:21642. PubMed
  10. Zhang Z, et al. 2016. PLoS One. 11: 0162700. PubMed
  11. Li L, et al. 2017. Stem Cell Research & Therapy . 10.1186/s13287-017-0565-7. PubMed
  12. Hu F, et al. 2017. Genomics. 10.1016/j.ygeno.2017.05.004. PubMed
  13. Pal B, et al. 2017. Nat Commun.. 10.1038/s41467-017-01560-x. PubMed
  14. Luo L, et al. 2018. Int J Mol Med. 41:51. PubMed
  15. Zhao J, et al. 2018. Stem Cell Reports. 10:180. PubMed
  16. Ge Q, et al. 2018. Mol Med Rep. 17:1667. PubMed
  17. Guo J, et al. 2018. Int J Mol Med. 41:1976. PubMed
  18. Li CJ, et al. 2018. J Clin Invest. 128:5251. PubMed
Publication Library
RRID
AB_312882 (BioLegend Cat. No. 102205)
AB_312883 (BioLegend Cat. No. 102206)

Antigen Details

Structure
Integrin family, 130 kD
Distribution

Leukocytes, endothelial cells, smooth muscle, epithelial cells

Function
Adhesion
Ligand/Receptor
Extracellular matrix
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Noto K, et al. 1995. Int. Immunol. 7:835.
2. Springer TA. 1990. Nature 346:425.

Gene ID
16412 View all products for this Gene ID 24511 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
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