FITC anti-mouse I-Ab Antibody

Pricing & Availability
Clone
KH74 (See other available formats)
Other Names
MHC class II
Isotype
Mouse (BALB/c) IgG2a, κ
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Product Citations
publications
KH74
C57BL/6 splenocytes stained with KH74 FITC
  • KH74
    C57BL/6 splenocytes stained with KH74 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
115305 50 µg $90
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Description

The KH74 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The KH74 antibody cross-reacts with the I-Af, I-Ar, I-As, and I-Av haplotypes.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse b haplotype, Cross-reacts with the I-A f,r,s,v haplotypes
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/10 mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen tissue sections. Does not react with other haplotypes (e.g., a, d, k, n, p, q, u).

Application References

(PubMed link indicates BioLegend citation)
  1. Hasenkrug KJ, et al. 1987. Immunogenetics 25:136.
  2. Pappo J, et al. 1999. Infect. Immun. 67:337.
Product Citations
  1. Wang Z 2018. Immunity. 49:80. PubMed
Publication Library
RRID
AB_313634 (BioLegend Cat. No. 115305)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand Receptor
CD3/TCR, CD4
Cell Type
B cells, T cells, Antigen-presenting cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID
UniProt
View information about I-Ab on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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