FITC anti-mouse H-2Kk Antibody

Pricing & Availability
36-7-5 (See other available formats)
Other Names
MHC class I
Mouse (A.TL) IgG2a, κ
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Product Citations
C3H/He mouse splenocytes were stained with H-2Kk (clone 36-7-5) FITC (filled histogram), or mouse IgG2a, κ FITC isotype control (open histogram).
  • 36-7-5_FITC_H2Kk_Antibody_FC_1_111213
    C3H/He mouse splenocytes were stained with H-2Kk (clone 36-7-5) FITC (filled histogram), or mouse IgG2a, κ FITC isotype control (open histogram).
  • 36-7-5_FITC_H2Kk_Antibody_FC_2_111213
    BALB/C mouse splenocytes were stained with H-2Kk (clone 36-7-5) FITC (filled histogram), or mouse (A.TL) IgG2a, κ FITC isotype control (open histogram).
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Cat # Size Price Quantity Avail. Save
114905 50 µg $80
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The 36-7-5 antibody reacts with the H-2Kk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kk haplotype. H-2Kk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 36-7-5 antibody does not react with alloantigens form other haplotypes (b, d, q), but weakly cross-reacts with splenocytes from SJL/Hsd mice.

Product Details
Technical data sheet

Product Details

Mouse k haplotype, Weakly cross-reacts with SJL/Hsd mice splenocytes
Antibody Type
Host Species
A.AL mouse splenocytes
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, and immunohistochemistry2,3 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 114914).

Does not react with alloantigens of other haplotypes (e.g., b, d, q).

Application References

(PubMed link indicates BioLegend citation)
  1. Goudeau B, et al. 2003. P. Natl. Acad. Sci. USA 100:15800.
  2. Johnson RM. 2004. Infect. Immun. 72:3951.
  3. Ardehali A, et al. 1995. Circulation 92:450.
  4. Day EB, et al. 2011. J. Immunol. 186:6319. PubMed.
Product Citations
  1. Day E, et al. 2011. J Immunol. 186:6319. PubMed
Publication Library
AB_313612 (BioLegend Cat. No. 114905)

Antigen Details

MHC class I

Nucleated cells of the H-2kk haplotype

Antigen presentation
Ligand Receptor
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. Ian A, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14972 View all products for this Gene ID
View information about H-2Kk on

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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