FITC anti-mouse H-2Kk Antibody

Pricing & Availability
Clone
AF3-12.1 (See other available formats)
Other Names
MHC class I
Isotype
Mouse (BALB/c) IgG1, κ
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Product Citations
publications
AF3-12dot1_FITC_122007
C3H mouse splenocytes stained with AF3-12.1 FITC
  • AF3-12dot1_FITC_122007
    C3H mouse splenocytes stained with AF3-12.1 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
116304 500 µg $250
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Description

The AF3-12.1 antibody reacts with the H-2Kk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kk haplotype. H-2Kk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF3-12.1 antibody does not cross-react with other haplotypes (b, d, f, j, p, q, r, s, u, v).

Product Details
Technical data sheet

Product Details

Reactivity
Mouse k haplotype
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
A/J mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
Does not react with other haplotypes (e.g. b, d, f, j, p, q, r, s, u, v).
Application References

(PubMed link indicates BioLegend citation)
  1. Wasowska B, et al. 2001. Transplantation 71:727.
  2. Yamamoto M, et al. 1998. J. Immunol. 160:2188.
RRID
AB_313719 (BioLegend Cat. No. 116304)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2kk haplotype

Function
Antigen presentation
Ligand Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. Ian A, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kk on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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