FITC anti-mouse H-2Kb/H-2Db Antibody

Pricing & Availability
Clone
28-8-6 (See other available formats)
Other Names
MHC class I
Isotype
Mouse (C3H) IgG2a, κ
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Product Citations
publications
28-8-6_FITC_H-2KH-2D_Antibody_FC_050713
C57BL/6 mouse splenocytes were stained with H-2Kb/H-2Db (clone 28-8-6) FITC (filled histogram) or mouse IgG2a, κ isotype control FITC (open histogram).
  • 28-8-6_FITC_H-2KH-2D_Antibody_FC_050713
    C57BL/6 mouse splenocytes were stained with H-2Kb/H-2Db (clone 28-8-6) FITC (filled histogram) or mouse IgG2a, κ isotype control FITC (open histogram).
See FITC spectral data
Cat # Size Price Quantity Avail. Save
114605 50 µg $80
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114606 500 µg $250
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Description

The 28-8-6 antibody reacts with the H-2Kb and H-2Db MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Db haplotype. H-2Kb/H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2Dd MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse b haplotype, Cross-reacts with H-2Dd MHC class I alloantigen
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H.SW mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt)
  2. Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC)
  3. Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed.
  4. Shao H, et al. 2005. J. Immunol. 175:1851. (FC)
Product Citations
  1. Bui J, et al. 2006. J Immunol. 176:905. PubMed
  2. Ueda N, et al. 2006. Int Immunol. 1.720138889. PubMed
  3. He X, et al. 2009. Stem Cells. 27:1487. PubMed
  4. Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed
  5. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  6. Bruna A, et al. 2016. Cell. 167: 260-274. PubMed
Publication Library
RRID
AB_313596 (BioLegend Cat. No. 114605)
AB_313597 (BioLegend Cat. No. 114606)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kb/H-2Db haplotype

Function
Antigen presentation
Ligand Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Ozato K, et al. 1981. J. Immunol. 126:317.
2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447.
3. Evans GA, et al. 1992. Nature 300:755.

Gene ID
14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt
View information about H-2K b,H-2D b on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05/07/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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