FITC anti-mouse CD40 Antibody

Pricing & Availability
Clone
HM40-3 (See other available formats)
Other Names
Bp50, TNFRSF5
Isotype
Armenian Hamster IgM
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Product Citations
publications
HM40-3_FITC_090507
C57BL/6 mouse splenocytes stained with HM40-3 FITC
  • HM40-3_FITC_090507
    C57BL/6 mouse splenocytes stained with HM40-3 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
102905 50 µg $70
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102906 500 µg $240
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Description

CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
(BALB/c x NZB)F1 mouse-derived lymphoma WEHI 231
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was conjugated with FITC under optimal conditions, and is at >85% purity. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD40 ligand binding1, stimulation of mouse B cell and dendritic cell proliferation2-6,9, and immunohistochemistry7 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102908).

Application References

(PubMed link indicates BioLegend citation)
  1. Kawano T, et al. 1997. Science 278:1626. (Block)
  2. Kaneko Y, et al. 1996. Eur. J. Immunol. 26:3061. (Costim)
  3. Kashiwada M, et al. 1996. Eur. J. Immunol. 26:1451. (Costim)
  4. Inaba M, et al. 1995. Eur. J. Immunol. 25:1244. (Costim)
  5. Akiba H, et al. 1999. J. Immunol. 162:7058. (Costim)
  6. Ridge JP, et al. 1998. Nature 393:474. (Costim)
  7. Morelli AE, et al. 2000. Transplantation 69:2647. (IHC)
  8. Ma XT, et al. 2006. Cancer Research 66:1169.
  9. Yu X, et al. 2006. J. Biol. Chem. 281:15505. (Costim)
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
RRID
AB_312948 (BioLegend Cat. No. 102905)
AB_312949 (BioLegend Cat. No. 102906)

Antigen Details

Structure
TNFR superfamily, 48 kD
Distribution

B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors

Function
Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
Ligand Receptor
CD40L (CD154)
Cell Type
B cells, Epithelial cells, Macrophages, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.

Gene ID
21939 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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