FITC anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
Ave. Rating
8 reviews
Product Citations
publications
145-2C11_FITC_021606
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) FITC and CD127 (clone SB/199) PE.
  • 145-2C11_FITC_021606
    C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) FITC and CD127 (clone SB/199) PE.
See FITC spectral data
Cat # Size Price Quantity Avail. Save
100305 50 µg $25
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100306 500 µg $90
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Koning B, et al. 2006. Int Immunol. 1.403472222. PubMed
  2. Schulteis R, et al. 2008. Blood. 112:4905. PubMed
  3. Kanaya T, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 295:273. PubMed
  4. Patnode M, et al. 2013. Glycobiology. 23:381. PubMed
  5. Xu M, et al. 2013. J Immunol. 190:5436. PubMed
  6. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  7. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  8. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  9. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  10. Yin Q, et al. 2015. PLoS One. 10: 0137808. PubMed
  11. Ma Y, et al. 2015. J Immunol. 195: 3769 - 3780. PubMed
  12. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  13. Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed
  14. Fornari T, et al. 2015. PLoS One. 10: 0142688. PubMed
  15. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  16. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
  17. Miki Y, et al. 2016. J Biol Chem. 291: 15588 - 15601. PubMed
  18. L M, et al. 2016. Brain. 139: 1939-1957. PubMed
  19. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  20. Matsumura K, et al. 2016. J Immunol. 197: 3233 - 3244. PubMed
  21. Allegrezza M, et al. 2016. Cancer Res . 76: 6253 - 6265. PubMed
  22. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  23. Schofield L, et al. 2017. BMC Med. 10.1186/s12916-017-0883-8. PubMed
  24. Bertrand F, et al. 2017. Nat Commun.. 10.1038/s41467-017-02358-7. PubMed
  25. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  26. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  27. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  28. Liu Y 2018. Cancer cell. 33:480. PubMed
  29. Best SA 2018. Cell metabolism. 27:935. PubMed
  30. Lin JR 2018. eLife. 7: e31657. PubMed
  31. Cong J 2018. Cell metabolism. 28:243. PubMed
  32. Knolle MD 2018. Frontiers in immunology. 9:2232. PubMed
Publication Library
RRID
AB_312670 (BioLegend Cat. No. 100305)
AB_312671 (BioLegend Cat. No. 100306)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3e on UniProt.org

Related FAQs

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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