- G8.8 (See other available formats)
- Other Names
- CD326, EGP40, MIC18, TROP1, KSA
- Rat IgG2a, κ
- Ave. Rating
- 2 reviews
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EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.Product Details
- Host Species
- TE-71 thymic epithelial cell line
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4.
(PubMed link indicates BioLegend citation)
- Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
- Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
- Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
- Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
- Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
- Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
- Ishii K, et al. 2012. Development. 139:1734. PubMed
- Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
- Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
- Ding X, et al. 2015. Cancer Res. 75:330. PubMed
- Furstenberg R, et al. 2011. Am J Physiol Gastrointest Liver Physiol. 300:G409. PubMed
- King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
- Saenz F, et al. 2014. PLoS One. 9:97666. PubMed
- Cleary A, et al. 2014. Nature. 508:113. PubMed
- Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
- Bittner-Eddy P, et al. 2016. J Immunol. 197: 1435 - 1446. PubMed
- Atapattu L, et al. 2016. J Exp Med. 213: 1741 - 1757. PubMed
- Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
- Matern M, et al. 2017. Sci Rep. 7:42079. PubMed
- Wlodarska M, et al. 2017. Cell Host Microbe. 10.1016/j.chom.2017.06.007. PubMed
AB_1134106 (BioLegend Cat. No. 118207)
AB_1134107 (BioLegend Cat. No. 118208)
- 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
- Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.
- Mediates calcium-independent homophilic cell-cell adhesion.
- CD326 displays hemophilic binding.
- Ligand Receptor
- CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
- Antigen References
1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.
- Gene ID
- 17075 View all products for this Gene ID
- View information about CD326 on UniProt.org
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