FITC anti-mouse CD31 Antibody

Pricing & Availability
Clone
390 (See other available formats)
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
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Product Citations
publications
390_FITC_080607
C57BL/6 mouse splenocytes stained with 390 FITC
  • 390_FITC_080607
    C57BL/6 mouse splenocytes stained with 390 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
102405 50 µg $75
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102406 500 µg $240
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Yoshida Y, et al. 2015. J Mol Cell Cardiol. 85: 183-198. PubMed
  2. Wen A, et al. 2012. J Vis Exp. 69: 4352. PubMed
  3. Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed
  4. Sullivan D, Watson R, Muller W 2016. Am J Physiol Heart Circ Physiol. 311: H621 - H632. PubMed
  5. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  6. Stubelius A, et al. 2017. Immunobiology. 10.1016/j.imbio.2017.05.007. PubMed
  7. Yip HYK, et al. 2018. PLoS One. 3. PubMed
  8. Hepler C 2018. eLife. 7: e39636. PubMed
  9. Huang Z, et al. 2017. Cell Metab. 26:493. PubMed
  10. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  11. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
  12. Kotla S, et al. 2019. JCI Insight. 4:9. PubMed
  13. Diebold LP, et al. 2019. Nat Metab. 1:158. PubMed
  14. Yong T, et al. 2019. Nat Commun. 10:3838. PubMed
Publication Library
RRID
AB_312900 (BioLegend Cat. No. 102405)
AB_312901 (BioLegend Cat. No. 102406)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 07/19/2013

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