FITC anti-human Perforin Antibody

Pricing & Availability
Clone
dG9 (See other available formats)
Other Names
PRF1, P1, PFP, HPLH2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
DG9
Whole blood lymphocytes stained intracellularly with dG9 FITC
  • DG9
    Whole blood lymphocytes stained intracellularly with dG9 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
308103 25 tests $95
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308104 100 tests $220
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Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Bovine (Cow, Cattle)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified granules from the human lymphoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone dG9 primarily recognizes perforin associated with cytotoxic granules9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.


Does not cross-react with mouse1.

Application References

(PubMed link indicates BioLegend citation)
  1. Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
  2. Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
  3. Watanabe N, et al. 1997. Blood 90:3662.
  4. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
  5. Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
  6. Chen H, et al. 2005. J. Immunol. 175:591.
  7. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
  8. Wood SM, et al. 2009. Blood 114:4117. PubMed
  9. Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.
Product Citations
  1. Strauss L, et al. 2009. PLoS One. 4:e5994. PubMed
  2. Cellerai C, et al. 2011. PLoS One. 6:e18164. PubMed
  3. Chen Y, et al. 2012. Cytokine. 58:40. PubMed
  4. Lünemann A, et al. 2013. J Immunol. 191:4989. PubMed
  5. Petri R, et al. 2017. Stem Cell Reports. 10.1016/j.stemcr.2017.06.020. PubMed
  6. de Jonge K, et al. 2019. Sci Rep. 9:4487. PubMed
Publication Library
RRID
AB_314701 (BioLegend Cat. No. 308103)
AB_314702 (BioLegend Cat. No. 308104)

Antigen Details

Structure
70 kD
Distribution

CTL, NK (cytoplasmic granules)

Function
Mediates targeted cell lysis
Cell Type
T cells, NK cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.

Gene ID
5551 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/14/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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