FITC anti-human IL-6 Antibody

Pricing & Availability
Clone
MQ2-13A5 (See other available formats)
Other Names
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Isotype
Rat IgG1, κ
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Product Citations
publications
MQ2-13A5_FITC_031808
LPS-stimulated (6 hours) human peripheral blood monocytes surface stained with CD14 APC and intracellular stained with MQ2-13A5 FITC
  • MQ2-13A5_FITC_031808
    LPS-stimulated (6 hours) human peripheral blood monocytes surface stained with CD14 APC and intracellular stained with MQ2-13A5 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
501103 25 tests $80
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501105 50 µg $105
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501104 100 tests $200
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Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-7- expressed, recombinant human IL-6
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
µg sizes: 0.5 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

ELISA or ELISPOT Capture1-3,7: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1-3,5,6: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Additional applications (for the relevant formats) include: intracellular flow cytometry10

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
  3. Gaines Das R, et al. 1993. J. Immunol. Methods 160:147.
  4. Enriquez J, et al. 2002. Adv. Perit Dial. 18:177.
  5. Zou JP, et al. 1999. J. Immunol. 162:4882.
  6. Wyant TL, et al. 1999. Infect. Immun. 67:1338.
  7. Lesmeister MJ, et al. 2005. Reprod. Biol. Endocrinol. 3:74.
  8. Terasaka Y, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:2441 PubMed
  9. Girndt M, et al. 1998. J. Am. Soc. Nephrol. 9:1689.
Product Citations
  1. Said A, et al. 2014. J Immunol. 193:6135. PubMed
  2. Ehrenstein M, et al. 2015. J Immunol. 194:2482. PubMed
  3. Peric Z, et al. 2015. Biol Blood Marrow Transplant. 21: 1431-1436. PubMed
  4. Lin JR 2018. eLife. 7: e31657. PubMed
Publication Library
RRID
AB_315151 (BioLegend Cat. No. 501103)
AB_315153 (BioLegend Cat. No. 501105)
AB_315152 (BioLegend Cat. No. 501104)

Antigen Details

Structure
Neuropoietic cytokine family; 26 kD (Mammalian)
Bioactivity
Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets
T cells, B cells, hepatocytes, cholinergic neurons
Receptors
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Int. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. van Oers M, et al. 1993. Ann. Hematol. 66:219.

Regulation
Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, and IL-17; downregulated by glucocorticoids IL-4 and TGF-β
Gene ID
3569 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 07/11/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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