FITC anti-human CLEC1B (CLEC2) Antibody

Pricing & Availability
Clone
AYP1 (See other available formats)
Other Names
C-type lectin domain family 1 member B, CLEC2B, PRO1384, QDED721
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
AYP1_FITC_CLEC1B_Antibody_021317
Human platelets were stained with CLEC1B (clone AYP1) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
  • AYP1_FITC_CLEC1B_Antibody_021317
    Human platelets were stained with CLEC1B (clone AYP1) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
See FITC spectral data
Cat # Size Price Quantity Avail. Save
372007 25 tests $100
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372008 100 tests $235
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Description

C-type lectin domain family 1 member B (CLEC1B, CLEC2) is a 32kD, type II transmembrane protein, highly expressed on platelets and megakaryocytes. CLEC1B is involved in platelet activation and aggregation, and mediates the capture of HIV-1 by platelets. CLEC1B contains one hem-immunoreceptor tyrosine-based activation motif (hemITAM) though which interact with Syk and PLC-g. The known ligands of CLEC1B are rhodocytin and podoplanin.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular region of CLEC1B (amino acids 68-229).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation; inhibition of podoplanin and rhodocytin-mediated platelet activation.

Application References

(PubMed link indicates BioLegend citation)
  1. Gitz E, et al. 2014. Blood 124:2262.
RRID
AB_2650868 (BioLegend Cat. No. 372007)
AB_2650869 (BioLegend Cat. No. 372008)

Antigen Details

Structure
Type II transmembrane protein, 32 kD, homodimer, 1 hemITAM motif.
Distribution
Platelets, megakaryocytes; low expression on myeloid cells.
Function
Mediates platelet activation and aggregation; facilitates capture of HIV by platelets.
Interaction
Syk, PLC-g.
Ligand/Receptor
Rhodocytin, podoplanin.
Cell Type
Platelets, Megakaryocytes, Dendritic cells
Biology Area
Immunology
Antigen References

1. Manne BK, et al. 2015. J. Biol. Chem. 290:11557.
2. Hughes CE, et al. 2013. J. Biol. Chem. 288:5127.
3. Parguiña AF, et al. 2012. Blood 120:e117-26.
4. Osada M, et al. 2012. J. Biol. Chem. 287:22241.

Gene ID
51266 View all products for this Gene ID
UniProt
View information about CLEC1B on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/15/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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