FITC anti-human CD49b Antibody

Pricing & Availability
Clone
P1E6-C5 (See other available formats)
Other Names
α2 integrin, VLA-2 α chain, Integrin α2 chain
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1E6-C5_FITC_CD49b_Antibody_FC_090513.jpg
Human peripheral blood platelets were stained with CD49b (clone P1E6-C5) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
  • P1E6-C5_FITC_CD49b_Antibody_FC_090513.jpg
    Human peripheral blood platelets were stained with CD49b (clone P1E6-C5) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
See FITC spectral data
Cat # Size Price Quantity Avail. Save
359305 25 tests $95
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359306 100 tests $195
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Description

CD49b is a 170 kD transmembrane protein, also known as α2 integrin, VLA-2 α chain, Integrin α2 and GPIa. It associates with CD29 (β1 integrin) to form VLA-2, a collagen and laminin receptor on many cell types including monocytes, platelets, activated T cells, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts. CD49b has been reported to interact with F-actin and matrix metalloproteinase 1. CD49b is a platelet alloantigen and has been associated with neonatal alloimmune thrombocytopenia. Deficiencies in this protein have been associated with hemorrhagic disorders.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HT1080 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: in vitro blocking activity1,2, immunoprecipitation3, and ELISA4. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional studies (Cat. No. 359304).

Application References

(PubMed link indicates BioLegend citation)
  1. Hirsch MS, et al. 1997. Dev. Dyn. 210:249. (Block)
  2. Sawhney RS, et al. 2006. J. Biol. Chem. 281:8497. (Block)
  3. Lee SA, et al. 2009. Carcinogensis. 30:1872. (IP)
  4. Zßrate S, et al. 2004. J. Virol. 78:10839. (ELISA)
Product Citations
  1. Lin JR 2018. eLife. 7: e31657. PubMed
Publication Library
RRID
AB_2562530 (BioLegend Cat. No. 359305)
AB_2562531 (BioLegend Cat. No. 359306)

Antigen Details

Structure
Integrin alpha chain family, type I membrane protein, alpha chain of heterodimeric integrin receptor, 170 kD; associates with CD29 to form collagen, laminin receptor
Distribution

Broadly expressed on many cell types including B cells, monocytes, platelets, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts

Function
With CD29; binds collagen and laminin
Ligand Receptor
Forms heterodimer with integrin β1 (CD29) which is a receptor for laminin and collagen
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kaplan C, et al. 1991. Br. J. Haematol. 78:425.
2. Kiefel V, et al. 1991. Vox Sang. 60:244.
3. Nieuwenhuis HK, et al. 1985. Nature 318:470.
4. Takada Y and Helmer ME. 1989. J. Cell Biol. 109:397.

Gene ID
3673 View all products for this Gene ID
UniProt
View information about CD49b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/13/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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