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FITC anti-human CD338 (ABCG2) Antibody

Pricing & Availability
Clone
5D3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
ABCG2, CDw338, Bcrp1, MXR
Isotype
Mouse IgG2b, κ
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Product Citations
publications
5D3_FITC_061708.jpg
ABCG2 transfected cells stained with 5D3 FITC
  • 5D3_FITC_061708.jpg
    ABCG2 transfected cells stained with 5D3 FITC
Compare all formats See FITC spectral data
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332014 100 tests $229
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Description

CD338, known as ABCG2 (BCRP1), is a multi-drug resistance (MDR) family protein that is a member of ATP binding cassette (ABC) transporters. It is highly expressed on primitive ‘side population’ (SP) stem cells. This SP phenotype is based on the efflux of fluorescent dyes such as Rhodamine 123 and Hoechst 33342. The SP cells express low or undetectable levels of CD34.  In the bone marrow, about 0.05% population of cells displays the low fluorescence and are highly enriched for repopulating cells.  The highest levels of BCRP1 mRNA expression have been seen in KDR+ human stem cells.  The expression of ABCG2 appears to be highly conserved as it has been identified in various species.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

This clone is listed under the following US Patent Numbers:
6,313,277; 7,541,437; 7,655,755; 7,741,455

Application References

(PubMed link indicates BioLegend citation)
  1. Matalia H, 2012. Br J Ophthalmol. PubMed
  2. 2. Zhou S, et al. 2001. Nat. Med. 7:1028. (FC)
  3. Abbott BL, et al. 2002. Blood 100:4594. (FC)
  4. Huang FF, et al. 2104. PloS One. 9:88298. PubMed
Product Citations
  1. Gazi U, et al. 2011. J Biol Chem. 286:7822. PubMed
  2. Jin J, et al. 2014. PLoS One. 9:104753. PubMed
  3. Kim JB, et al. 2017. Mol Med Rep. 16:453. PubMed
  4. Zhao Y, et al. 2021. Oncol Lett. 364:21. PubMed
RRID
AB_1134191 (BioLegend Cat. No. 332014)

Antigen Details

Distribution

primitive 'sidepopulation' (SP) stem cells, KDR+ human stem cells

Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Kim M, et al. 2002. Clin. Cancer Res. 8:228.
2. Scharenber CW, et al. 2002. Blood 99:50712.
3.Goodell MA, et al. 1997. Nat. Med. 3:133745.
4. Bunting KD. 2002. Stem Cells. 20:11.

Gene ID
9429 View all products for this Gene ID
UniProt
View information about CD338 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/17/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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