FITC anti-CD247 (TCRζ, CD3ζ) Antibody

Pricing & Availability
6B10.2 (See other available formats)
Other Names
CD247, CD3zeta
Mouse IgG1 κ
Ave. Rating
6 reviews
Product Citations
Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) PE then intracellular stained with 6B10.2 FITC
  • 6B10dot2_FITC_050109.jpg
    Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) PE then intracellular stained with 6B10.2 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
644103 25 µg $105
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644104 100 µg $265
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The invariant TCR zeta chain is a member of the CD3 complex associated with the clonotypic alpha/beta TCR heterodimer. The disulfide-linked TCR zeta homodimers transmit signals following TCR ligation. Loss of TCR zeta expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Human TCR zeta chain aa38-54
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhang Z, et al. 2007. Blood 109:4328. (Block)
  2. Gorman CL, et al. 2008. J. Immunol. 180:1060.
  3. Jenson WA, et al. 1997. Eur J. Immunol. 27:707.
  4. Mao H, et al. 2010. J. Virol. 84:4148. PubMed
  5. Hwang I, et al. 2012. Int Immunol. 24:793. PubMed.
Product Citations
  1. Sade-Feldman M, et al. 2016. Clin Cancer Res. 22(23):5661-5672. PubMed
  2. Memmer S, et al. 2016. J Biol Chem. 291(49):25427-25438. PubMed
Publication Library
AB_2244275 (BioLegend Cat. No. 644103)
AB_2072959 (BioLegend Cat. No. 644104)

Antigen Details

163 amino acid long protein with a short extracellular domain of only nine amino acids conserved between mouse and human. There are 6-7 intracellular tyrosines that are potential phosphorylation sites.
T cells and NKT cells. Reported also positive on CD16+ human NK cells.
Mediating signal transduction after TCR receptor engagement.
ZAP70, TCR alpha/beta
Cell Type
T cells, NKT cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
TCRs, CD Molecules
Antigen References

1.Weissman A, et al. 1988. P. Natl. Acad. Sci. USA 85:9709

Gene ID
919 View all products for this Gene ID
View information about CD247 on

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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