ELISA MAX™ Deluxe Set SARS CoV-2 Nucleocapsid Protein

Pricing & Availability
Regulatory Status
RUO
Other Names
Nucleocapsid (N) protein, SARS-CoV-2 N Antigen, Nucleocapsid phosphoprotein, Nucleoprotein, SARS-CoV-2 Nucleocapsid Protein
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ELISA-MAX_Deluxe_SARS_Cov_Nucleocapsid_112922
  • ELISA-MAX_Deluxe_SARS_Cov_Nucleocapsid_112922
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448004 5 plates $373
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Description

The nucleocapsid protein is an integral component of SARS-CoV-2 virus, and it is highly conserved among the coronaviruses. The protein's main functions are interaction with viral RNA and the membrane protein during assembly, as well as nucleocapsid structure of the viral core. These functions increase the efficiency of viral transmission and assembly. In SARS and other viral infections, nucleocapsid protein levels in patient serum have been shown to be a descriptive marker of the disease progression. Nucleocapsid protein can be detected in patient serum early in disease onset, prior to antiviral IgG or IgM, and detection levels tend to stay high into the infection.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • SARS-CoV-2 Nucleocapsid Capture Antibody (200X)
  • SARS-CoV-2 Nucleocapsid Detection Antibody (200X)
  • SARS-CoV-2 Nucleocapsid Protein Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)
  • Blocking Buffer A (2X)
  • Assay Buffer H

Product Details

Verified Reactivity
SARS-CoV-2
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
21.4 pg/mL
Standard Range
125 - 8000 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 11/29/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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