- Other Names
- RNA pol beta prime, rpoC, tabB, DNA-directed, RNAP subunit beta prime, Transcriptase subunit beta prime, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit alpha, Transcriptase subunit alpha, pez, phs, sez
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DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. α subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme. The β subunit is the second-largest subunit, and is encoded by the rpoB gene. The β’ (β prime) subunit contains structures crucial for transcription, including the sites for ribonucleotide addition and catalysis.
For additional antigen details, please refer to individual product datasheets.
- Kit Contents
Specificity Clone Size Reactivity Isotype MW (kD) E. coli RNA Polymerase β Prime NT73 25 µg E. coli, Enterics, Borellia burgdorferi, Salmonella Mouse IgG1 155 E. coli RNA Polymerase β NT63 25 µg E. coli Mouse IgG2a 150 E. coli RNA Polymerase α 4RA2 25 µg E. coli, Widely among Gram-negative bacteria, some Gram-positive bacteria Mouse IgG1 36 E. coli RNA Polymerase β 8RB13 25 µg E. coli, Bordetella, Chlamydia, Myxococcus, Salmonella, Vibrio Mouse IgG1 150
* For detailed information about each specificity, please follow the provided links.
- Please refer to individual product datasheets for details.
- All antibodies in this kit were purified by affinity chromatography.
- Storage & Handling
- Upon receipt, store undiluted at 2-8°C.
WB - Quality tested
- Recommended Usage
Each lot of antibodies in this kit is quality control tested by Western Blotting. For Western blotting, the suggested uses of these reagents are as follows:
Anti-E. coli RNA Polymerase β Prime (clone NT73): 1:1000-1:4000 (0.25-1ug/mL)
Anti-E. coli RNA Polymerase β (clone NT63): 1:2500-1:100000 (0.005-0.2ug/mL)
Anti-E. coli RNA Polymerase α (clone 4RA2): 1:500-1:5000 (0.1-1.0 ug/mL)
Anti-E. coli RNA Polymerase β (clone 8RB13): 1:2500-1:100000 (0.005-0.2ug/mL)
It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
For validated or reported applications for these antibodies, please see individual product datasheets.
Clone NT73 recognizes epitope 1295-1417 (SLAELLNAGLGGS), and is extremely useful for purifying holoenzymes from enterics. It does not cross-react with mycobacterial smegmatis RNAP. This antibody is used for purifying Softag 1 epitope tagged protein expressed in eukaryotic systems.
Clone NT63 recognizes epitope 922-1090 of β subunit of the E. coli RNA polymerase and can be used in WB analysis.
Clone 8RB13 is extremely useful for detecting (WB) or purifying (IP) core RNAP from a broad range of bacteria.
Clone 4RA2 specifically recognizes α subunit of the E. coli RNA polymerase.