Loading...

DyLight™ 488 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-DyLight™ 488
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
405218 100 µg $118
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Streptavidin binds to biotin with high affinity. DyLight™ 488-conjugated Streptavidin is useful for detecting biotinylated antibodies. The excitation of DyLight™ 488 by 493 nm laser light induces a fluorescence emission maximum of 518 nm.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
Streptavidin is conjugated with DyLight™ 488 under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F, IHC-P
Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the DyLight™ 488 conjugation. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Streptavidin-DyLight™ 488 is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-DyLight™ 488 is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Ramos TN, et al. 2011. J. Immunol. 186:6657. PubMed.
  2. Yan ZX, et al. 2012. Am J Physiol Heart Circ Phyciol. 302:215. PubMed
  3. Chen WP, et al. 2014. Cardiovasc Res. 105:44. PubMed
Product Citations
  1. Ghosh D, et al. 2020. J Cell Sci. . PubMed
  2. Chen W, et al. 2014. Cardiovasc Res. 105:44. PubMed
  3. Fischer A, et al. 2015. Gut. 101136/gutjnl-2015-310022. PubMed
  4. Ramos T, et al. 2011. J Immunol. 186:6657. PubMed
  5. Yan Z, et al. 2012. Am J Physiol Heart Circ Physiol. 302:H215. PubMed
  6. Ghosh D, et al. 2020. J Cell Sci. 133:00:00. PubMed
  7. Zhang J, et al. 2022. Int J Biol Sci. 18:6068. PubMed
  8. Li X, et al. 2021. Eur J Immunol. 51:2452. PubMed

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 06/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account