DRAQ5™

Pricing & Availability
Other Names
Live/Dead Staining, Dead cell exclusion dye, Cell viability dye
Ave. Rating
1 reviews
Product Citations
publications
DRAQ5_1_IF_072815
HeLa cells were fixed with 1% paraformaldehyde (PFA) and blocked with 5% fetal bovine serum for 30 minutes at room temperature. Then the cells were stained with 10 µg/ml of EGFR (clone AY13) Brilliant Violet 421™ (blue) for three hours at room temperature. Nuclei were counterstained with 1:250 dilution of DRAQ5 (red). The image was captured with a 20 x objective.
  • DRAQ5_1_IF_072815
    HeLa cells were fixed with 1% paraformaldehyde (PFA) and blocked with 5% fetal bovine serum for 30 minutes at room temperature. Then the cells were stained with 10 µg/ml of EGFR (clone AY13) Brilliant Violet 421™ (blue) for three hours at room temperature. Nuclei were counterstained with 1:250 dilution of DRAQ5 (red). The image was captured with a 20 x objective.
  • DRAQ5_2_FC_042414
    To study cell cycle analysis, C57BL/6 mouse bone marrow cells were stained with DRAQ5™ and acquired on a 633 nm laser-equipped flow cytometer. The above histogram shows DRAQ5™ detected with the 715 nm filter (PE/Cy7 channel).
See DRAQ5™ spectral data See IF/IHC/FC data...
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424101 50 µl $185
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Description

DRAQ5™ is a far-red emitting, anthraquinone compound that stains nuclei in live cells. It is permeant to live cells and thus can be used for cell cycle analysis and/or staining of nucleated cells. It is optimally excited at 568 nm, 633 nm, and 647nm and can be detected using 695LP, 715LP, and 780LP filters. DRAQ5™ can also be used in cell imaging and is a good replacement for DAPI, as it does not get excited by UV or Violet lasers and is sub-optimally excited by the 488 nm laser. DRAQ5™ can be combined with FITC, PE, and other UV or violet excitable dyes for multi-color analysis.

Product Details
Technical data sheet

Product Details

Preparation
DRAQ5™ is supplied at 50 µl per vial (5 mM).
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
DRAQ5™ should be stored between 2°C and 8°C upon receipt.
Application

FC, ICFC - Quality tested
IF, IHC-P - Validated

Recommended Usage

DRAQ5™ is a trademark of Biostatus Limited.

Application Notes

DRAQ5™ is a cell-permeant DNA binding anthraquinone dye that intercalates between A-T bases of dsDNA. Due to its cell permeability, this dye is useful for assessing DNA content and cell cycle but is not suitable to be used as a viability dye. In microscopy applications, DRAQ5™ is also useful as a nuclear counterstain for both live and fixed specimens. It is a far-red emitting dye with optimal excitation/ emission peaks of 633nm/ 695nm, respectively, (when intercalated into DNA) that can be detected in the Alexa Fluor® 647, APC and Alexa Fluor® 700 channels. For use in cell cycle analysis, the optimal concentration must be determined for each cell type. Also, due to the wide emission spectrum and the brightness of DRAQ5™ off the 633nm red laser and the dim emission intensity of the APC-Cy7 or APC-Fire™ 750 fluorophores, flow cytometric panels should be optimized to determine that including DRAQ5™ in a panel is compatible with the use of the APC-Cy7 or APC-Fire™ 750 channel as well.

Protocol for DNA staining using DRAQ5™:

1. Perform surface staining following protocol of choice.

2. Wash cells twice with phosphate buffered saline (PBS). Sodium azide interferes with DRAQ5™ staining, thus it is recommended to stain in PBS (without calcium, magnesium, or sodium azide) or culture medium.

3. Dilute DRAQ5™ to required concentration. We recommend titrating the reagent to determine optimal concentration for cells of interest. Good results in flow cytometry and microscopy applications have been observed using a 1:200 to 1:1000 dilution.

4. Incubate at room temperature, preventing exposure to light, for 10-15 minutes. Subsequent washing can help make the cell cycle profile more distinct but is not absolutely necessary since DRAQ5™ is a fluorogenic reagent.

5. Analyze cells on a cytometer equipped with the 633 red laser. If performing cell cycle analysis, detection in the Alexa 700 channel with a 680LP or 715LP filter might help with resolution of the emission peaks.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. Sándor N, et al. 2016. PLoS One. 11: 0163120. PubMed
  2. Webb A, et al. 2017. BMC Cancer . 10.1186/s12885-017-3418-y. PubMed
  3. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
Publication Library

Antigen Details

Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Neuroscience, Cell Proliferation and Viability
Antigen References

1. Smith PJ, et al. 1999. J. Immunol. Methods. 229:131.
2. Smith PJ, et al. 2000. Cytometry. 40:280.
3. Yuan CM, et al. 2004. Cytometry B. Clin. Cytom. 58:47.

Gene ID
NA

Related FAQs

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Go To Top Version: 6    Revision Date: 01/16/2017

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