Direct-Blot™ HRP anti-SOX2 Antibody

Pricing & Availability
Clone
14A6A34 (See other available formats)
Other Names
SRY-related HMG-box gene 2, SRY (sex determining region Y)-box 2, MCOPS3, ANOP3
Isotype
Mouse IgG1, κ
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Product Citations
publications
14A6A34_DBHRP_SOX2_Antibody_032117_updated
Total cell lysate from HeLa (lane 1)and NTERA2 (lane 2) (15 µg/lane) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti-SOX2 (clone 14A6A34) antibody or 1:1,000, 1:2,000 and 1:10,000 Direct-Blot™ HRP anti-SOX2 (clone 14A6A34) antibody. Purified proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control. Lane M is the molecular weight ladder.
  • 14A6A34_DBHRP_SOX2_Antibody_032117_updated
    Total cell lysate from HeLa (lane 1)and NTERA2 (lane 2) (15 µg/lane) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti-SOX2 (clone 14A6A34) antibody or 1:1,000, 1:2,000 and 1:10,000 Direct-Blot™ HRP anti-SOX2 (clone 14A6A34) antibody. Purified proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control. Lane M is the molecular weight ladder.
Cat # Size Price Quantity Avail. Save
656115 100 µL $290
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Description

SOX2 is the most studied member of SRY-related box transcription factor family. It binds to target genes through its highly conserved HMG box domain. Inactivation of the SOX2 gene causes lethality during embryonic development. SOX2 knockdown in embryonic stem cells results in their differentiation. Co-expression of SOX with OCT4, MYC, and KLF4 is sufficient to reprogram somatic cells to induced pluripotent stem cells (iPSCs), which exert similar characteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial for the self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2 has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cycle arrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting that SOX2 is involved in activating genes associated with tumor progression.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Host Species
Mouse
Immunogen
Full length human SOX recombinant protein
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:2,000-1:20,000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2650805 (BioLegend Cat. No. 656115)

Antigen Details

Structure
317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding
Distribution

Nucleus

Function
Transcription factor that regulates the expression of the genes involved in embryonic development
Interaction
Interacts with FGFR1, SOX3, and ZSCAN10
Cell Type
Mesenchymal Stem Cells, Embryonic Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Antigen References

1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228.
2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725.
3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772.
4. Jeong CH, et al. 2010. Stem Cells 28:2141.
5. Xiang R, et al. 2011. Br. J. Cancer 104:1410.
6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426.

Gene ID
6657 View all products for this Gene ID
UniProt
View information about SOX2 on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 03/07/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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