Direct-Blot™ HRP anti-Hsp70 Antibody

Pricing & Availability
Clone
W27 (See other available formats)
Other Names
Heat shock protein 70
Isotype
Mouse IgG2a, λ
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Product Citations
publications
W27_DirectBlotHRP_Hsp70_WB_Image_010616
10 µg of total protein extract from HeLa cells was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-HSP70 (clone W27). Proteins were visualized using chemiluminescence detection.
  • W27_DirectBlotHRP_Hsp70_WB_Image_010616
    10 µg of total protein extract from HeLa cells was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-HSP70 (clone W27). Proteins were visualized using chemiluminescence detection.
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648005 100 µL $290
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Description

Hsp70 is a member of the Hsp family of highly conserved proteins that play a role in a number of important functions. Their synthesis is increased upon environmental stress. They function by acting as molecular chaperones, facilitating protein refolding and preventing protein aggregation. It has been reported that extracellular Hsp70 also exhibits immunomodulatory effects on innate and acquired immunity.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Hsp70 protein from HeLa cells.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

RRID
AB_2566707 (BioLegend Cat. No. 648005)

Antigen Details

Structure
Hsp proteins are the most conserved proteins present in both prokaryotes and eukaryotes. Human HSp70 contains multiple isoforms. Hsp70s consisit of three structural domains, a 44Kd amino-terminal ATPase domain (NBD), followed by an 18 Kd substrate
Distribution

Ubiquitous, cytoplasmic and nuclear

Function
Molecular chaperrones assisting in the folding of proteins and preventing protein aggregation
Biology Area
Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance
Antigen References

1. Sharma D, et al. 2009. Protein Peptide Lett. 16:571.
2. Johnson JD, et al. 2006. J. Leuk. Biol. 79:425.
3. Bukau B, et al. 1998. Cell 92:351.

Gene ID
3308 View all products for this Gene ID
UniProt
View information about Hsp70 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04/21/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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