Direct-Blot™ HRP anti-HDAC3 Antibody

Pricing & Availability
Clone
W15097A (See other available formats)
Other Names
Histone deacetylase 3, HD3, SMAP45, RPD3-2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
W15097A_DB-HRP_HDAC3_Antibody_WB_042017
Total cell lysate (15µg protein) from HeLa cells was resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1:1000 purified anti-HDAC3 or 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti-HDAC3 Antibody (upper) or loading control GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for the purified antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the MW ladder.
  • W15097A_DB-HRP_HDAC3_Antibody_WB_042017
    Total cell lysate (15µg protein) from HeLa cells was resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1:1000 purified anti-HDAC3 or 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti-HDAC3 Antibody (upper) or loading control GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for the purified antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the MW ladder.
  • W15097A_DB-HRP_HDAC3_Antibody_WB_2_071918
    Total cell lysates (15 µg protein) from HeLa cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with A) 1:1000 dilution and B) 1:10000 dilution of Direct-Blot™ HRP anti-HDAC3 Antibody, clone W15097A (upper panel) or 1:4000 dilution of Direct-Blot™ HRP anti-GAPDH antibody, clone W17079A (lower panel) as a loading control. Proteins were visualized by chemiluminescence detection. Lane M: molecular weight ladder.
Cat # Size Price Quantity Avail. Save
685205 100 µl $290
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Description

HDAC3, also known as RPD3-2, HDAC-3, and SMAP45, is a histone deacetylase and is responsible for the deacetylation of lysine residues on core histones and other non-histone substrates. The deacetylation of histones is important for transcriptional regulation, cell cycle progression, and other developmental related functions. HDAC3 represses gene expression by participating in the transcriptional repressor activity of BCL6, by deacetylating H3K27 on enhancer elements. It is also related to participating in transcriptional regulation through binding to YY1, which is a a zinc-finger transcription factor.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human HDAC3 recombinant protein (405-424 a.a.) conjugated to KLH.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:10000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2687106 (BioLegend Cat. No. 685205)

Antigen Details

Structure
428 amino acids with a predicted molecular weight of 49 kD. It belongs to the class I histone deacetylase family.
Distribution

Nucleus.

Function
HDAC3 is a deacetylase responsible for the deacetylation of lysine resides on core histones and other non-histone proteins.
Interaction
Forms heterodimer with YY1. Interacts with HDAC7, 9, 10, DACH1, DAXX, BCOR, MJD2A/JHDM3A, NRIP1, PRDM6, and SRY.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Hua G, et al. 2016. Proc Natl Acad Sci USA. 113:E626.
2. Choi HK, et al. 2015. Nat. Commun. 6:8225.
3. Kim WY, et al. 2015. Int. J. Mol. Med. 35(1):202-10.
4. McHugh CA, et al. 2015. Nature 521:232.
5. Sun Z, et al. 2013. Mol. Cell. 52:769.
6. Sun Z, et al. 2012. Nat. Med. 18:934.

Gene ID
8841 View all products for this Gene ID
UniProt
View information about HDAC3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 04/21/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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