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Direct-Blot™ HRP anti-GAPDH Antibody

Pricing & Availability
Clone
W17079A (See other available formats)
Regulatory Status
RUO
Other Names
glyceraldehyde-3-phosphate dehydrogenase
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17079A_DB-HRP_GAPDH_Antibody_1_WB_062218
Western Blot of human lysate using film imaging system. Whole cell lysates (15 µg protein) from HeLa and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:4000 Direct-Blot™ HRP anti-GAPDH antibody, clone W17079A (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Proteins were visualized using chemiluminescence detection with film imaging system. Lane M: MW ladder.
  • W17079A_DB-HRP_GAPDH_Antibody_1_WB_062218
    Western Blot of human lysate using film imaging system. Whole cell lysates (15 µg protein) from HeLa and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:4000 Direct-Blot™ HRP anti-GAPDH antibody, clone W17079A (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Proteins were visualized using chemiluminescence detection with film imaging system. Lane M: MW ladder.
  • W17079A_DB-HRP_GAPDH_Antibody_2_WB_022824
    Western Blot of human lysate using digital imaging system. Whole cell lysates (15 µg total protein) from HeLa cells was resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with indicated dilutions of Direct-Blot™ HRP anti-GAPDH (clone W17079A) or Purified anti-GAPDH (clone W17079A) (Cat. No. 607902) as a reference. Proteins were visualized by HRP Goat anti rat-IgG (Cat. No.405405) for Purified anti-GAPDH. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as chemiluminescence detection agent with a high sensitivity WB membrane imaging system. Ponceau S staining was used as loading control (lower). Lane M: Molecular weight marker
  • W17079A_DB-HRP_GAPDH_Antibody_3_WB_022824
    Western Blot of mouse lysate using digital imaging system. Whole cell lysates (15 µg total protein) from NIH/3T3 cells was resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with indicated dilutions of Direct-Blot™ HRP anti-GAPDH (clone W17079A) or Purified anti-GAPDH (clone W17079A) (Cat. No. 607902). Proteins were visualized by HRP Goat anti rat-IgG (Cat. No.405405) for Purified anti-GAPDH. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as chemiluminescence detection agent with a high sensitivity WB membrane imaging system. Ponceau S staining was used as loading control (lower). Lane M: Molecular weight marker
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607903 25 µL $101
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607904 100 µL $340
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Description

GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein (161-335 a.a.)
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 µg per ml (1:4000 - 1:16000 dilution). For high sensitivity WB membrane imaging systems, the suggested concentration is 0.01  µg per ml (1:50000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone weakly reacts with rat GAPDH.

Product Citations
  1. Zhou Q, et al. 2020. J Invest Dermatol. . PubMed
  2. Moujalled D, et al. 2021. Cell Death Dis. 12:133. PubMed
RRID
AB_2734504 (BioLegend Cat. No. 607903)
AB_2734505 (BioLegend Cat. No. 607904)

Antigen Details

Structure
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution

Ubiquitously expressed, locate in cytoplasm and perinuclear region.

Function
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Interaction
Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2.
Biology Area
Cell Biology, Western Blot Controls
Antigen References
  1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
  2. Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460.
  3. Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527.
  4. Zheng L, et al. 2003. Cell. 114:255.
  5. Wang Q, et al. 2005. FASEB J. 19:869.
  6. Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405.
Gene ID
5590 View all products for this Gene ID
UniProt
View information about GAPDH on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/22/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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