Direct-Blot™ HRP anti-E. coli RNA Sigma 70 Antibody

Pricing & Availability
Clone
2G10 (See other available formats)
Other Names
RNA pol sigma 70, rpoD, sigma D, RNA Polymerase
Isotype
Mouse IgG2b
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Product Citations
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2G10_DBHRP_Ecoli_RNASigma70_Antibody_092216
15 µg of total protein extract from E. coli was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP (DB-HRP) anti-E. coli RNA Sigma 70 (clone 2G10). Proteins were visualized using chemiluminescence detection.
  • 2G10_DBHRP_Ecoli_RNASigma70_Antibody_092216
    15 µg of total protein extract from E. coli was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP (DB-HRP) anti-E. coli RNA Sigma 70 (clone 2G10). Proteins were visualized using chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
663205 100 µl $290
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Description

RNA polymerase transcribes DNA to synthesize RNA. Prokaryotes contain a single RNA polymerase- core RNA polymerase. RNA polymerase sigma 70 (also known as RNA pol sigma 70 and rpoD) is a part of a family of sigma factor subunits that are required for specific promoter recognition and initiation. RNA pol sigma 70 is the major housekeeping sigma factor responsible for RNA polymerase recognition out of the majority of promoters necessary to transcribe bacterial genes which are expressed during expansion.

Product Details
Technical data sheet

Product Details

Reactivity
E. coli, Chlamydia trachomatis, Mycobacterium smegmatis, Staphylococcus aureus, Bordetella pertussis, Rhodobacter sphaeroides, Vibrio parahaemolyticus, Pseudomonas aeruginosa, Agrobacterium tumefaciens, Streptococcus faecalis, Arthrobacter globiformis
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Previously item# WP004 from Neoclone.

Clone 2G10 is extremely useful for identifying the major sigma factor from a variety of bacteria. It does not react with Borellia burgdorferi. Clone 2G10 recognizes epitope 470-486.

Additional reported applications (for the relevant formats) include: functional assay and immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Breyer MJ, et al. 1997. J. Bacterial 179:1404.
  2. Lesley SA, et al. 1989. J. Biol. Chem. 264:3794.
  3. Strickland MS, et al. 1988. Biochemistry 27:5755.
RRID
AB_2629596 (BioLegend Cat. No. 663205)

Antigen Details

Structure
Sigma 70 (σ70) is a 70 kD transcription factor of E. coli RNA polymerase.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
947567 View all products for this Gene ID
UniProt
View information about Sigma 70 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/22/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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