Direct-Blot™ HRP anti-Cytokeratin (pan reactive) Antibody

Pricing & Availability
Clone
C-11 (See other available formats)
Other Names
Cytokeratin, Keratin
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cdash11_DBHRP_Cytokeratin_panreactive_Antibody_062916
15 µg of total protein extract from HeLa was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP anti-Cytokeratin (pan reactive) antibody (clone C-11). Proteins were visualized using chemiluminescence detection. Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control.
  • Cdash11_DBHRP_Cytokeratin_panreactive_Antibody_062916
    15 µg of total protein extract from HeLa was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP anti-Cytokeratin (pan reactive) antibody (clone C-11). Proteins were visualized using chemiluminescence detection. Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control.
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628609 100 µl $290
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Description

Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.

Product Details
Technical data sheet

Product Details

Reactivity
Recognizes human cytokeratin 4, 5, 6, 8, 10, 13, and 18.
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Keratin enriched fraction from human epidermoid carcinoma.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry.

Application References

(PubMed link indicates BioLegend citation)
  1. Kovarik J. 1988. Int. J. Cancer Suppl. 3:50.
  2. Bartek J, et al. 1991. J. Pathol. 164:215.
  3. Chernyavsky A I, 2007. J. Biol. Chem. doi:10.1074/jbc.M611365200. PubMed
  4. Comito G, et al. 2012. Cancer Lett. 324:31. PubMed
  5. Vojt-òsek B, et al. 1989. Folia Biol (Praha). 35:373. (reactivity with rat, dog, sheep, pig, cow)
  6. Dekaney CM, et al. 2005. Gastroenterology 129:1567. (ICC)
RRID
AB_2617030 (BioLegend Cat. No. 628609)

Antigen Details

Structure
Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits
Distribution

Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus

Function
Intermediate filament protein involved with the cytoskeleton.
Interaction
Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3875 View all products for this Gene ID
UniProt
View information about Cytokeratin (pan reactive) on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/29/2016

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