- W16197A (See other available formats)
- Regulatory Status
- Other Names
- ACTB, Actin, cytoplasmic 1, Beta-actin
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).Product Details
- Verified Reactivity
- Human, Mouse, Rat, Zebrafish, Drosophila
- Antibody Type
- Host Species
- Human ACTB peptide (1-13 a.a.).
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.01 µg per ml (1:10,000 - 1:100,000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
- Application Notes
Reactivity to Drosophila was only verified with the purified format.
- Product Citations
AB_2810693 (BioLegend Cat. No. 664803)
AB_2728496 (BioLegend Cat. No. 664804)
- 375 amino acids with a predicted molecular weight of 42 kD.
- Actins are highly conserved proteins that are involved in cell motility.
- Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Antigen References
1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
- Gene ID
- 60 View all products for this Gene ID
- View information about beta-actin on UniProt.org
|Direct-Blot™ HRP anti-β-actin||W16197A||WB|
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