Cy5 Streptavidin

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Other Names
SAv-Cy5
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FrancescaMazzoni.jpg
Unpassaged live mouse primary retinal pigment epithelial cells engulfed biotinylated photocreceptor outer segments (POS) coupled to Cy5 Streptavidin (red). Cells were subsequently stained with Flash Phalloidin™ Green 488 (green) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Francesca Mazzoni from Fordham University.
  • FrancescaMazzoni.jpg
    Unpassaged live mouse primary retinal pigment epithelial cells engulfed biotinylated photocreceptor outer segments (POS) coupled to Cy5 Streptavidin (red). Cells were subsequently stained with Flash Phalloidin™ Green 488 (green) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Francesca Mazzoni from Fordham University.
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405209 100 µg $175.00
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Cy5 is useful for detecting biotinylated antibodies. The excitation of Cy5 by 625-650 nm laser light induces a fluorescence emission maximum of 670 nm.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Human, Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with Cy5 under optimal conditions. The solution is free of unconjugated Cy5.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-Cy5 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this Streptavidin-Cy5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Cy5 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Cy® and CyDye® are registered trademarks of GE Healthcare.

Application Notes

Streptavidin-Cy5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of streptavidin-Cy5 is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsu HC, et al. 2007. J. Immunol. 178:5357.
  2. Biegler KA, et al. 2012. Cancer Prev Res (Phila). 5:1173. PubMed
  3. Cheng JY, et al. 2014. Cancer Res. 74:6856. PubMed
  4. Biegler K, et al. 2012. Cancer Prev Res. 5:1173. PubMed
  5. Cheng J, et al. 2014. Cancer Res. 74:6856. PubMed
  6. Dedeoglu B, et al. 2016. PLoS One. 11: 0150826. PubMed
  7. Müller-Durovic B, et al. 2016. J Immunol. 197: 2891 - 2899. PubMed
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