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Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP

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Other Names
Fc Receptor Blocking Solution
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Cell-Vive_CD_Human_TruStain_FcX_GMP_083022
THP-1 cells were treated with Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP followed by staining with an irrelevant PE mouse IgG2a, κ Isotype (clone MOPC-173, purple histogram). Non-specific binding of the same antibody on untreated cells is shown in red.
  • Cell-Vive_CD_Human_TruStain_FcX_GMP_083022
    THP-1 cells were treated with Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP followed by staining with an irrelevant PE mouse IgG2a, κ Isotype (clone MOPC-173, purple histogram). Non-specific binding of the same antibody on untreated cells is shown in red.
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420510 100 tests $275
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Description

Human Fc receptors (FcRs) are molecules that regulate the immune responses. FcRs are expressed by several hematopoietic cells. Due to FcRs-mediated Ig Fc binding, false positive or false negative results of immunofluorescent staining could occur. Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP is specially formulated to block the FcR-involved unwanted staining without interfering with the antibody-mediated specific staining of human cells. Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP is compatible with flow cytometric staining with anti-human CD16 (clone 3G8), CD32 (clone FUN-2), and CD64 (clone 10.1) antibodies. Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP contains ultra-low endotoxin levels (<0.01EU/µg of protein) and is tested negative for mycoplasma and microbial growths.
This GMP product is for research and further ex vivo bioprocessing use only.

Product Details
Technical data sheet

Product Details

Formulation
Serum-Free, Defined, containing no preservatives. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Storage & Handling
Store between 2°- 8°C. Handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

5 μl of Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP per million cells in 100 μl staining buffer.

Application Notes

This buffer contains specialized human IgG. It is not recommended to be used for staining human IgG. Handle as biohazard agent. Add 5 μl of Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP per million cells in 100 μl staining buffer, mix and incubate at room temperature for 5-10 minutes prior to staining with antibody of interest. It is not necessary to wash cells between these blocking and immunostaining steps.

Antigen Details

Antigen References
  1. Bruhns P, 2012. Blood. 119:5640-9
  2. Patera A, et al. 2016. J Leukoc Biol. 100:1239-1254. PubMed
  3. Kreutmair S, et al. 2021. Immunity. 54:1578-1593. PubMed
Gene ID
NA

Related FAQs

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Go To Top Version: 3    Revision Date: 06/26/2023

For research and further manufacturing use. Not for diagnostic or therapeutic procedures. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to reverse engineer functionally similar materials without written approval of BioLegend. By use of these products you accept the terms and conditions of any and all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research and further manufacturing use only and not intended for human or animal diagnostic or therapeutic use.

 

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