Brilliant Violet 711™ anti-human CD31 Antibody

Pricing & Availability
Clone
WM59 (See other available formats)
Workshop
V P025
Other Names
PECAM-1, EndoCAM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
WM59_BV711_CD31_Antibody_092117
Human peripheral blood granulocytes were stained with CD31 (clone WM59) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram).
  • WM59_BV711_CD31_Antibody_092117
    Human peripheral blood granulocytes were stained with CD31 (clone WM59) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram).
See Brilliant Violet 711™ spectral data
Cat # Size Price Quantity Avail. Save
303135 25 tests $195
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303136 100 tests $405
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Description

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, and blocking of platelet aggregation3. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).

The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotinylated O92E4 (Cat. No. 536604) antibody as the detection antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
  2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
  3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
  4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
  6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  8. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
  9. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  10. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed
RRID
AB_2716212 (BioLegend Cat. No. 303135)
AB_2716213 (BioLegend Cat. No. 303136)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

Function
Cell adhesion, signal transduction
Ligand/Receptor
CD38
Cell Type
Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. DeLisser H, et al. 1994. Immunol. Today 15:490.
  2. Newman P, 1997. J. Clin. Invest. 99:3.
  3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 09/21/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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