Brilliant Violet 711™ anti-human CD2 Antibody

Pricing & Availability
Clone
RPA-2.10 (See other available formats)
Workshop
IV T085
Other Names
LFA-2, T11, SRBC-R
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
RPA-2dot10_BV710_CD2_Antibody_022519.png
Human peripheral blood lymphocytes were stained with CD3 PE and CD2 (clone RPA-2.10) Brilliant Violet 711™ (left), or Mouse IgG1, κ Brilliant Violet 711™ isotype control (right).
  • RPA-2dot10_BV710_CD2_Antibody_022519.png
    Human peripheral blood lymphocytes were stained with CD3 PE and CD2 (clone RPA-2.10) Brilliant Violet 711™ (left), or Mouse IgG1, κ Brilliant Violet 711™ isotype control (right).
See Brilliant Violet 711™ spectral data
Cat # Size Price Quantity Avail. Save
300231 25 tests $195
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300232 100 tests $395
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Description

CD2 is a 50 kD type I transmembrane glycoprotein also known as LFA-2, T11, and sheep red blood cell receptor (SRBC-R). This immunoglobulin superfamily member is expressed on thymocytes, T lymphocytes, NK cells, and thymic B cell subsets. The major ligand for CD2 is CD58 (also known as LFA-3). CD2 has also been reported to bind CD48, CD59, and CD15. CD2 plays a critical role in alternative T cell activation, T cell signaling, and cell-cell adhesion.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Swine (Pig, Porcine)
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 711™ and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and blocking of T cell activation2. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 300239 & 300240).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Aversa G, et al. 1987. Transplant. Proc. 19:277. (Block)
  3. Zaretsky AG, et al. 2009. J. Exp Med. 206:991. (IHC) PubMed
  4. Perona-Wright G, et al. 2010. Nat. Immunol. 11:520. (FC) PubMed
  5. Thummler K, et al. 2010. J. Leukoc. Biol. 88:1041.
  6. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 50 kD
Distribution

T cells, NK cells, thymocytes, and thymic B cell subsets

Function
T cell activation, adhesion
Ligand/Receptor
CD58 (LFA-3), CD48, CD59, CD15
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Bell G, et al. 1995. J. Immunol. 155:2805.
2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Moingeon P, et al. 1989. Immunol. Rev. 111:111.

Gene ID
914 View all products for this Gene ID
UniProt
View information about CD2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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